Evidence for redifferentiation of human chondrocytes grown on a hyaluronan-based biomaterial (HYAFF®11): Molecular, immunohistochemical and ultrastructural analysis

Brunella Grigolo, Gina Lisignoli, Anna Piacentini, Mauro Fiorini, Pietro Gobbi, Giovanni Mazzotti, Manuela Duca, Alessandra Pavesio, Andrea Facchini

Research output: Contribution to journalArticle

Abstract

Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage repair. Such scaffolds should provide a preformed three-dimensional shape and prevent cells from escaping into the articular cavity. Furthermore, these constructs should have sufficient mechanical strength to facilitate handling in a clinical setting and stimulate the uniform spreading of cells and their phenotype redifferentiation. The aim of this study was to verify the ability of HYAFF®11, a recently developed hyaluronic-acid-based biodegradable polymer, to support the growth of human chondrocytes and to maintain their original phenotype. This capability was assessed by the evaluation of collagen types I, II and aggrecan mRNA expression. Immunohistochemical analyses were also performed to evaluate collagen types I, II and proteoglycans synthesis. A field emission in lens scanning microscopy was utilized to verify the interactions between the cells and the biomaterial. Our data indicate that human chondrocytes seeded on HYAFF®11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration for the therapeutic potential of HYAFF®11 as a delivery vehicle in a tissue-engineered approach towards the repair of articular cartilage defects.

Original languageEnglish
Pages (from-to)1187-1195
Number of pages9
JournalBiomaterials
Volume23
Issue number4
DOIs
Publication statusPublished - Feb 15 2002

Keywords

  • Chondrocytes
  • Collagen
  • Hyaluronan
  • Proteoglycans
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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