Evidence for redifferentiation of human chondrocytes grown on a hyaluronan-based biomaterial (HYAFF®11): Molecular, immunohistochemical and ultrastructural analysis

Brunella Grigolo, Gina Lisignoli, Anna Piacentini, Mauro Fiorini, Pietro Gobbi, Giovanni Mazzotti, Manuela Duca, Alessandra Pavesio, Andrea Facchini

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage repair. Such scaffolds should provide a preformed three-dimensional shape and prevent cells from escaping into the articular cavity. Furthermore, these constructs should have sufficient mechanical strength to facilitate handling in a clinical setting and stimulate the uniform spreading of cells and their phenotype redifferentiation. The aim of this study was to verify the ability of HYAFF®11, a recently developed hyaluronic-acid-based biodegradable polymer, to support the growth of human chondrocytes and to maintain their original phenotype. This capability was assessed by the evaluation of collagen types I, II and aggrecan mRNA expression. Immunohistochemical analyses were also performed to evaluate collagen types I, II and proteoglycans synthesis. A field emission in lens scanning microscopy was utilized to verify the interactions between the cells and the biomaterial. Our data indicate that human chondrocytes seeded on HYAFF®11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration for the therapeutic potential of HYAFF®11 as a delivery vehicle in a tissue-engineered approach towards the repair of articular cartilage defects.

Original languageEnglish
Pages (from-to)1187-1195
Number of pages9
JournalBiomaterials
Volume23
Issue number4
DOIs
Publication statusPublished - Feb 15 2002

Fingerprint

Collagen Type II
Biocompatible Materials
Hyaluronic Acid
Chondrocytes
Collagen Type I
Collagen
Biomaterials
Aggrecans
Cartilage
Repair
Phenotype
Biodegradable polymers
Cell Shape
Articular Cartilage
Proteoglycans
Hyaluronic acid
Scaffolds
Cell Communication
Field emission
Lenses

Keywords

  • Chondrocytes
  • Collagen
  • Hyaluronan
  • Proteoglycans
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Evidence for redifferentiation of human chondrocytes grown on a hyaluronan-based biomaterial (HYAFF®11) : Molecular, immunohistochemical and ultrastructural analysis. / Grigolo, Brunella; Lisignoli, Gina; Piacentini, Anna; Fiorini, Mauro; Gobbi, Pietro; Mazzotti, Giovanni; Duca, Manuela; Pavesio, Alessandra; Facchini, Andrea.

In: Biomaterials, Vol. 23, No. 4, 15.02.2002, p. 1187-1195.

Research output: Contribution to journalArticle

Grigolo, Brunella ; Lisignoli, Gina ; Piacentini, Anna ; Fiorini, Mauro ; Gobbi, Pietro ; Mazzotti, Giovanni ; Duca, Manuela ; Pavesio, Alessandra ; Facchini, Andrea. / Evidence for redifferentiation of human chondrocytes grown on a hyaluronan-based biomaterial (HYAFF®11) : Molecular, immunohistochemical and ultrastructural analysis. In: Biomaterials. 2002 ; Vol. 23, No. 4. pp. 1187-1195.
@article{af64b92c9885464fa072192e1dd1be51,
title = "Evidence for redifferentiation of human chondrocytes grown on a hyaluronan-based biomaterial (HYAFF{\circledR}11): Molecular, immunohistochemical and ultrastructural analysis",
abstract = "Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage repair. Such scaffolds should provide a preformed three-dimensional shape and prevent cells from escaping into the articular cavity. Furthermore, these constructs should have sufficient mechanical strength to facilitate handling in a clinical setting and stimulate the uniform spreading of cells and their phenotype redifferentiation. The aim of this study was to verify the ability of HYAFF{\circledR}11, a recently developed hyaluronic-acid-based biodegradable polymer, to support the growth of human chondrocytes and to maintain their original phenotype. This capability was assessed by the evaluation of collagen types I, II and aggrecan mRNA expression. Immunohistochemical analyses were also performed to evaluate collagen types I, II and proteoglycans synthesis. A field emission in lens scanning microscopy was utilized to verify the interactions between the cells and the biomaterial. Our data indicate that human chondrocytes seeded on HYAFF{\circledR}11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration for the therapeutic potential of HYAFF{\circledR}11 as a delivery vehicle in a tissue-engineered approach towards the repair of articular cartilage defects.",
keywords = "Chondrocytes, Collagen, Hyaluronan, Proteoglycans, Tissue engineering",
author = "Brunella Grigolo and Gina Lisignoli and Anna Piacentini and Mauro Fiorini and Pietro Gobbi and Giovanni Mazzotti and Manuela Duca and Alessandra Pavesio and Andrea Facchini",
year = "2002",
month = "2",
day = "15",
doi = "10.1016/S0142-9612(01)00236-8",
language = "English",
volume = "23",
pages = "1187--1195",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - Evidence for redifferentiation of human chondrocytes grown on a hyaluronan-based biomaterial (HYAFF®11)

T2 - Molecular, immunohistochemical and ultrastructural analysis

AU - Grigolo, Brunella

AU - Lisignoli, Gina

AU - Piacentini, Anna

AU - Fiorini, Mauro

AU - Gobbi, Pietro

AU - Mazzotti, Giovanni

AU - Duca, Manuela

AU - Pavesio, Alessandra

AU - Facchini, Andrea

PY - 2002/2/15

Y1 - 2002/2/15

N2 - Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage repair. Such scaffolds should provide a preformed three-dimensional shape and prevent cells from escaping into the articular cavity. Furthermore, these constructs should have sufficient mechanical strength to facilitate handling in a clinical setting and stimulate the uniform spreading of cells and their phenotype redifferentiation. The aim of this study was to verify the ability of HYAFF®11, a recently developed hyaluronic-acid-based biodegradable polymer, to support the growth of human chondrocytes and to maintain their original phenotype. This capability was assessed by the evaluation of collagen types I, II and aggrecan mRNA expression. Immunohistochemical analyses were also performed to evaluate collagen types I, II and proteoglycans synthesis. A field emission in lens scanning microscopy was utilized to verify the interactions between the cells and the biomaterial. Our data indicate that human chondrocytes seeded on HYAFF®11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration for the therapeutic potential of HYAFF®11 as a delivery vehicle in a tissue-engineered approach towards the repair of articular cartilage defects.

AB - Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage repair. Such scaffolds should provide a preformed three-dimensional shape and prevent cells from escaping into the articular cavity. Furthermore, these constructs should have sufficient mechanical strength to facilitate handling in a clinical setting and stimulate the uniform spreading of cells and their phenotype redifferentiation. The aim of this study was to verify the ability of HYAFF®11, a recently developed hyaluronic-acid-based biodegradable polymer, to support the growth of human chondrocytes and to maintain their original phenotype. This capability was assessed by the evaluation of collagen types I, II and aggrecan mRNA expression. Immunohistochemical analyses were also performed to evaluate collagen types I, II and proteoglycans synthesis. A field emission in lens scanning microscopy was utilized to verify the interactions between the cells and the biomaterial. Our data indicate that human chondrocytes seeded on HYAFF®11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration for the therapeutic potential of HYAFF®11 as a delivery vehicle in a tissue-engineered approach towards the repair of articular cartilage defects.

KW - Chondrocytes

KW - Collagen

KW - Hyaluronan

KW - Proteoglycans

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=0037082712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037082712&partnerID=8YFLogxK

U2 - 10.1016/S0142-9612(01)00236-8

DO - 10.1016/S0142-9612(01)00236-8

M3 - Article

C2 - 11791922

AN - SCOPUS:0037082712

VL - 23

SP - 1187

EP - 1195

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 4

ER -