Cerebrospinal fluid (CSF) free light chains of κ or λ (FLC κ/λ) type were investigated by affinity mediated blotting technique (AMI) and ELISA in 28 patients of which nine with AIDS and Toxoplasma gondii encephalitis (AIDS, TE), 11 with AIDS with or without other CNS AIDS-related opportunistic infections (non-TE AIDS) and eight control patients with or without inflammatory neurological disorders (control group). CSF restricted oligoclonal FLC bands either of k or λ isotype or both were found by AMI in 18 (90%) out of 20 AIDS patients, while a CSF pattern predominantly characterized by FκLC rather than FλLC was observed in eight (88.8%) out of nine TE patients. No FLC components were detected in the matched sera of TE or non-TE AIDS patients or in the CSF and sera from control group. The anti-parasite-specific FκLC CSF/serum mean levels and the T. gondii-specific FκLC index values were found by ELISA to be significantly more elevated in TE patients when compared to non-TE AIDS or control group. These findings suggest that the increased production of T. gondii-specific FκLC could provide insights into pathogenesis of reactivated TE in immunocompromised patients and may have important diagnostic usefulness. Copyright (C) 2000 Elsevier Science B.V.
- Affinity mediated immunoblotting
- Cerebrospinal fluid
- Free light chains
- Toxoplasma gondii encephalitis
ASJC Scopus subject areas
- Clinical Neurology
- Immunology and Allergy