Evidence of COX-1 and COX-2 expression in Kaposi's sarcoma tissues

Luigi Rossiello, Eleonora Ruocco, Giuseppe Signoriello, Pietro Micheli, Monica Rienzo, Claudio Napoli, Raffaele Rossiello

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cyclooxygenases (COXs) are enzymes catalysing prostaglandin synthesis and are implicated in the carcinogenesis of some cancer types. In addition, an important role of these enzymes in herpesvirus infections was demonstrated and it has recently been proposed that COX-2 may participate in herpesvirus-induced neoplasia such as Kaposi's sarcoma (KS). To date no immunohistochemical study has been performed to determine the identification of COX-1 and COX-2 in KS. We have investigated 35 cases of classic KS and 27 cases of epidemic KS form in order to study the distribution and localisation of COXs. We have examined by immunohistochemistry the expression of COX-1 and COX-2 in classic and epidemic forms of KS also in relationship to the characteristic morphological phases (patch, plaque and nodular stage) of KS and cell localisation by double immunostaining. Moreover, we have obtained COX-1 and COX-2 expression by Western blot analysis. Our results establish that (a) COX-1 and COX-2 are overexpressed significantly in classic and epidemic KS compared with control skin tissues (P <0.01 and P > 0.03, respectively, for COX-1; P <0.01 and P > 0.03, respectively, for COX-2); (b) the extent and intensity staining for both COXs were higher in classic than in epidemic form of KS. Our data support the hypothesis that both COXs may be involved in the pathogenesis of KS.

Original languageEnglish
Pages (from-to)1232-1241
Number of pages10
JournalEuropean Journal of Cancer
Volume43
Issue number8
DOIs
Publication statusPublished - May 2007

Fingerprint

Cyclooxygenase 1
Kaposi's Sarcoma
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Herpesviridae Infections
Herpesviridae
Enzymes
Prostaglandins
Neoplasms
Carcinogenesis
Western Blotting
Immunohistochemistry
Staining and Labeling
Skin

Keywords

  • Cancer
  • COX
  • Kaposi's sarcoma

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Rossiello, L., Ruocco, E., Signoriello, G., Micheli, P., Rienzo, M., Napoli, C., & Rossiello, R. (2007). Evidence of COX-1 and COX-2 expression in Kaposi's sarcoma tissues. European Journal of Cancer, 43(8), 1232-1241. https://doi.org/10.1016/j.ejca.2007.03.016

Evidence of COX-1 and COX-2 expression in Kaposi's sarcoma tissues. / Rossiello, Luigi; Ruocco, Eleonora; Signoriello, Giuseppe; Micheli, Pietro; Rienzo, Monica; Napoli, Claudio; Rossiello, Raffaele.

In: European Journal of Cancer, Vol. 43, No. 8, 05.2007, p. 1232-1241.

Research output: Contribution to journalArticle

Rossiello, L, Ruocco, E, Signoriello, G, Micheli, P, Rienzo, M, Napoli, C & Rossiello, R 2007, 'Evidence of COX-1 and COX-2 expression in Kaposi's sarcoma tissues', European Journal of Cancer, vol. 43, no. 8, pp. 1232-1241. https://doi.org/10.1016/j.ejca.2007.03.016
Rossiello, Luigi ; Ruocco, Eleonora ; Signoriello, Giuseppe ; Micheli, Pietro ; Rienzo, Monica ; Napoli, Claudio ; Rossiello, Raffaele. / Evidence of COX-1 and COX-2 expression in Kaposi's sarcoma tissues. In: European Journal of Cancer. 2007 ; Vol. 43, No. 8. pp. 1232-1241.
@article{72b07b93e345427495479608e5051d93,
title = "Evidence of COX-1 and COX-2 expression in Kaposi's sarcoma tissues",
abstract = "Cyclooxygenases (COXs) are enzymes catalysing prostaglandin synthesis and are implicated in the carcinogenesis of some cancer types. In addition, an important role of these enzymes in herpesvirus infections was demonstrated and it has recently been proposed that COX-2 may participate in herpesvirus-induced neoplasia such as Kaposi's sarcoma (KS). To date no immunohistochemical study has been performed to determine the identification of COX-1 and COX-2 in KS. We have investigated 35 cases of classic KS and 27 cases of epidemic KS form in order to study the distribution and localisation of COXs. We have examined by immunohistochemistry the expression of COX-1 and COX-2 in classic and epidemic forms of KS also in relationship to the characteristic morphological phases (patch, plaque and nodular stage) of KS and cell localisation by double immunostaining. Moreover, we have obtained COX-1 and COX-2 expression by Western blot analysis. Our results establish that (a) COX-1 and COX-2 are overexpressed significantly in classic and epidemic KS compared with control skin tissues (P <0.01 and P > 0.03, respectively, for COX-1; P <0.01 and P > 0.03, respectively, for COX-2); (b) the extent and intensity staining for both COXs were higher in classic than in epidemic form of KS. Our data support the hypothesis that both COXs may be involved in the pathogenesis of KS.",
keywords = "Cancer, COX, Kaposi's sarcoma",
author = "Luigi Rossiello and Eleonora Ruocco and Giuseppe Signoriello and Pietro Micheli and Monica Rienzo and Claudio Napoli and Raffaele Rossiello",
year = "2007",
month = "5",
doi = "10.1016/j.ejca.2007.03.016",
language = "English",
volume = "43",
pages = "1232--1241",
journal = "European Journal of Cancer",
issn = "0959-8049",
publisher = "Elsevier Ltd",
number = "8",

}

TY - JOUR

T1 - Evidence of COX-1 and COX-2 expression in Kaposi's sarcoma tissues

AU - Rossiello, Luigi

AU - Ruocco, Eleonora

AU - Signoriello, Giuseppe

AU - Micheli, Pietro

AU - Rienzo, Monica

AU - Napoli, Claudio

AU - Rossiello, Raffaele

PY - 2007/5

Y1 - 2007/5

N2 - Cyclooxygenases (COXs) are enzymes catalysing prostaglandin synthesis and are implicated in the carcinogenesis of some cancer types. In addition, an important role of these enzymes in herpesvirus infections was demonstrated and it has recently been proposed that COX-2 may participate in herpesvirus-induced neoplasia such as Kaposi's sarcoma (KS). To date no immunohistochemical study has been performed to determine the identification of COX-1 and COX-2 in KS. We have investigated 35 cases of classic KS and 27 cases of epidemic KS form in order to study the distribution and localisation of COXs. We have examined by immunohistochemistry the expression of COX-1 and COX-2 in classic and epidemic forms of KS also in relationship to the characteristic morphological phases (patch, plaque and nodular stage) of KS and cell localisation by double immunostaining. Moreover, we have obtained COX-1 and COX-2 expression by Western blot analysis. Our results establish that (a) COX-1 and COX-2 are overexpressed significantly in classic and epidemic KS compared with control skin tissues (P <0.01 and P > 0.03, respectively, for COX-1; P <0.01 and P > 0.03, respectively, for COX-2); (b) the extent and intensity staining for both COXs were higher in classic than in epidemic form of KS. Our data support the hypothesis that both COXs may be involved in the pathogenesis of KS.

AB - Cyclooxygenases (COXs) are enzymes catalysing prostaglandin synthesis and are implicated in the carcinogenesis of some cancer types. In addition, an important role of these enzymes in herpesvirus infections was demonstrated and it has recently been proposed that COX-2 may participate in herpesvirus-induced neoplasia such as Kaposi's sarcoma (KS). To date no immunohistochemical study has been performed to determine the identification of COX-1 and COX-2 in KS. We have investigated 35 cases of classic KS and 27 cases of epidemic KS form in order to study the distribution and localisation of COXs. We have examined by immunohistochemistry the expression of COX-1 and COX-2 in classic and epidemic forms of KS also in relationship to the characteristic morphological phases (patch, plaque and nodular stage) of KS and cell localisation by double immunostaining. Moreover, we have obtained COX-1 and COX-2 expression by Western blot analysis. Our results establish that (a) COX-1 and COX-2 are overexpressed significantly in classic and epidemic KS compared with control skin tissues (P <0.01 and P > 0.03, respectively, for COX-1; P <0.01 and P > 0.03, respectively, for COX-2); (b) the extent and intensity staining for both COXs were higher in classic than in epidemic form of KS. Our data support the hypothesis that both COXs may be involved in the pathogenesis of KS.

KW - Cancer

KW - COX

KW - Kaposi's sarcoma

UR - http://www.scopus.com/inward/record.url?scp=34248191763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248191763&partnerID=8YFLogxK

U2 - 10.1016/j.ejca.2007.03.016

DO - 10.1016/j.ejca.2007.03.016

M3 - Article

VL - 43

SP - 1232

EP - 1241

JO - European Journal of Cancer

JF - European Journal of Cancer

SN - 0959-8049

IS - 8

ER -