Evidence of PAF-acether metabolic pathway activation in antigen challenge of upper respiratory airways

A. Miadonna, A. Tedeschi, B. Arnoux, A. Sala, C. Zanussi, J. Benveniste

Research output: Contribution to journalArticlepeer-review

Abstract

Lyso-PAF-acether and PAF-acether (formerly platelet-activating factor) were detected in nasal secretions from patients with hay fever who underwent local antigen challenge. Lyso-PAF release was observed in 12 of 13 patients, with a maximum (p <0.001) 5 min after stimulation and a progressive decrease during the first hour. PAF was detected in the 5-min postchallenge nasal washings from two of 13 subjects. After HPLC, this mediator was found in four of seven postchallenge nasal washings submitted to this procedure, with a peak 5 min and 10 min after provocation. Histamine analysis revealed a significant (p <0.001) but time-limited (5 min) release in nasal secretion. The pattern of immunoreactive leukotriene C4 showed a maximal peak (p <0.01) 5 min after allergen provocation, with raised levels for 20 min. Nasal stimulation with nebulized saline solution or grass pollens in healthy subjects and in patients suffering from allergic rhinitis caused by Dermatophagoides pteronyssinus was followed by no local mediator release. These data indicate that, in addition to histamine and peptide-leukotrienes, lyso-PAF and PAF are released in nasal secretions after local antigen stimulation in patients with hay fever, with a preponderance of lyso-PAF response. On the basis of these results, it is conceivable that these ether-phospholipids may be involved in allergic inflammation of human nasal airways.

Original languageEnglish
Pages (from-to)142-147
Number of pages6
JournalAmerican Review of Respiratory Disease
Volume140
Issue number1
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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