Evidence of poly(ADP-ribosylation) in the cockroach Periplaneta americana

Marco Denegri, Simonetta Lambiase, Chiara Donadoni, Laura Rossi, A. Ivana Scovassi

Research output: Contribution to journalArticle

Abstract

Poly(ADP-ribosylation) is a post-translational modification of nuclear proteins typical of most eukaryotic cells. This process participates in DNA replication and repair and is mainly regulated by two enzymes, poly(ADP-ribose) polymerase, which is responsible for the synthesis of polymers of ADP-ribose, and poly(ADP-ribose) glycohydrolase, which performs polymer degradation. The aim of this work was to investigate in the cockroach Periplaneta americana L. (Blattaria: Blattidae) the behaviour of poly(ADP-ribosylation). In particular, we addressed: (i) the possible modulation of poly(ADP-ribosylation) during the embryonic development; (ii) the expression of poly(ADP-ribose) polymerase and glycohydrolase in different tissues; and (iii) the role of poly(ADP-ribosylation) during spermatogenesis. In this work we demonstrated that: (i) as revealed by specific biochemical assays, active poly(ADP-ribose) polymerase and glycohydrolase are present exclusively in P. americana embryos at early stages of development; (ii) an activity carrying out poly(ADP-ribose) synthesis was found in extracts from testes; and (iii) the synthesis of poly(ADP-ribose) occurs preferentially in differentiating spermatids/spermatozoa. Collectively, our results indicate that the poly(ADP-ribosylation) process in P. americana, which is a hemimetabolous insect, displays catalytical and structural features similar to those described in the holometabolous insects and in mammalian cells. Furthermore, this process appears to be modulated during embryonic development and spermatogenesis. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)1045-1050
Number of pages6
JournalInsect Biochemistry and Molecular Biology
Volume30
Issue number11
DOIs
Publication statusPublished - 2000

Fingerprint

Periplaneta
Cockroaches
Periplaneta americana
ribose
Blattodea
NAD ADP-ribosyltransferase
Adenosine Diphosphate
glycosidases
Poly(ADP-ribose) Polymerases
Poly Adenosine Diphosphate Ribose
spermatogenesis
synthesis
Spermatogenesis
polymers
embryogenesis
Blattidae
Embryonic Development
Insects
Polymers
insects

Keywords

  • Insect development
  • PARG
  • PARP
  • Poly(ADP-ribosylation)
  • Spermatogenesis

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Evidence of poly(ADP-ribosylation) in the cockroach Periplaneta americana. / Denegri, Marco; Lambiase, Simonetta; Donadoni, Chiara; Rossi, Laura; Ivana Scovassi, A.

In: Insect Biochemistry and Molecular Biology, Vol. 30, No. 11, 2000, p. 1045-1050.

Research output: Contribution to journalArticle

Denegri, Marco ; Lambiase, Simonetta ; Donadoni, Chiara ; Rossi, Laura ; Ivana Scovassi, A. / Evidence of poly(ADP-ribosylation) in the cockroach Periplaneta americana. In: Insect Biochemistry and Molecular Biology. 2000 ; Vol. 30, No. 11. pp. 1045-1050.
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