Evidence of 18F-FCH uptake in human T98G glioblastoma cells

Federica Eleonora Buroni, Francesca Pasi, Marco Giovanni Persico, Lorenzo Lodola, Carlo Aprile, Rosanna Nano

Research output: Contribution to journalArticle

Abstract

Aim: Tumor and chemo/radiotherapy-damaged brain tissues are hardly distinguishable by conventional morphological imaging. 18F-FCH was compared against 18F-FDG in the T98G glioblastoma cell line with regard to their radiopharmaceutical uptake, in order to test its diagnostic power on brain tumor lesions. Materials and Methods: Equimolar amounts of 18F-FCH and 18F-FDG were added to human glioblastoma T98G cells and human dermal fibroblasts for 20, 40, 60, 90 and 120 min of incubation. Radiopharmaceutical uptake was expressed as a percentage of the administered dose. Cold choline was used for binding competition experiments. Results: In T98G cells 18F-FCH was taken-up in higher amounts than 18F-FDG after 60 min. In fibroblasts, uptake was lower than 1% for both radiopharmaceuticals. Cold choline reduced the uptake of FCH to 1% similarly to fibroblasts. Conclusion: Our results prove the efficacy of 18F-FCH as a promising tracer, better than 18F-FDG in establishing the tumor-tobackground ratio in brain tumors.

Original languageEnglish
Pages (from-to)6439-6444
Number of pages6
JournalAnticancer Research
Volume35
Issue number12
Publication statusPublished - Dec 1 2015

Keywords

  • F-fluorocholine
  • F-fluorodeoxyglucose
  • Glioma
  • Radiation injury
  • T98G human cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    Buroni, F. E., Pasi, F., Persico, M. G., Lodola, L., Aprile, C., & Nano, R. (2015). Evidence of 18F-FCH uptake in human T98G glioblastoma cells. Anticancer Research, 35(12), 6439-6444.