TY - JOUR
T1 - Evidence of the causal role of human papillomavirus type 58 in an oropharyngeal carcinoma
AU - Baboci, Lorena
AU - Boscolo-Rizzo, Paolo
AU - Holzinger, Dana
AU - Bertorelle, Roberta
AU - Biasini, Lorena
AU - Michel, Angelika
AU - Schmitt, Markus
AU - Spinato, Giacomo
AU - Bussani, Rossana
AU - Alemany, Laia
AU - Tirelli, Giancarlo
AU - Da Mosto, Maria Cristina
AU - Del Mistro, Annarosa
AU - Pawlita, Michael
PY - 2013
Y1 - 2013
N2 - Persistent human papillomavirus infection (HPV) is recognized as an important etiologic factor for a subset of head and neck squamous cell carcinomas (SCC), especially those arising from the oropharynx. Whereas HPV16 accounts for the majority of HPV DNA-positive oropharyngeal SCC, infections with other mucosal high-risk HPV types are quite rare and biological data demonstrating their causal involvement are insufficient. Here we present the first case of an oropharyngeal SCC driven by HPV type 58. A 69-year-old Caucasian woman presented with an enlarged and firm left tonsil. A computed tomography scan showed a left tonsillar mass, extending to the soft palate and the glossotonsillar sulcus. The patient underwent extended radical tonsillectomy and ipsilateral selective neck dissection. Pathology confirmed an infiltrating, poorly differentiated SCC of the left tonsil with node metastasis (pT2N1). Adjuvant external beam radiation therapy (60 Grays (Gy)) was administered. After 1 year of follow-up, the patient is well with no evidence of cancer recurrence. HPV analyses of the tumor tissue by BSGP5+/6+ -PCR/MPG, targeting 51 mucosal HPV types, showed single positivity for HPV type 58. Presence of HPV58 E6*I RNA demonstrated biological activity of the virus in the tumor tissue, and presence of serum antibodies to HPV58 oncoproteins E6 and E7 indicated presence of an HPV58-driven cancer. Overexpression of cellular protein p16§ssup§INK4a§esup§ and reduced expression of pRb, two cellular markers for HPV-induced cell transformation, were observed. Exons 4-10 of TP53 showed no mutations or polymorphisms. The presence of HPV58 as single HPV infection in combination with a broad variety of direct and indirect markers of HPV transformation provides comprehensive evidence that this oropharyngeal SCC was driven by HPV58.
AB - Persistent human papillomavirus infection (HPV) is recognized as an important etiologic factor for a subset of head and neck squamous cell carcinomas (SCC), especially those arising from the oropharynx. Whereas HPV16 accounts for the majority of HPV DNA-positive oropharyngeal SCC, infections with other mucosal high-risk HPV types are quite rare and biological data demonstrating their causal involvement are insufficient. Here we present the first case of an oropharyngeal SCC driven by HPV type 58. A 69-year-old Caucasian woman presented with an enlarged and firm left tonsil. A computed tomography scan showed a left tonsillar mass, extending to the soft palate and the glossotonsillar sulcus. The patient underwent extended radical tonsillectomy and ipsilateral selective neck dissection. Pathology confirmed an infiltrating, poorly differentiated SCC of the left tonsil with node metastasis (pT2N1). Adjuvant external beam radiation therapy (60 Grays (Gy)) was administered. After 1 year of follow-up, the patient is well with no evidence of cancer recurrence. HPV analyses of the tumor tissue by BSGP5+/6+ -PCR/MPG, targeting 51 mucosal HPV types, showed single positivity for HPV type 58. Presence of HPV58 E6*I RNA demonstrated biological activity of the virus in the tumor tissue, and presence of serum antibodies to HPV58 oncoproteins E6 and E7 indicated presence of an HPV58-driven cancer. Overexpression of cellular protein p16§ssup§INK4a§esup§ and reduced expression of pRb, two cellular markers for HPV-induced cell transformation, were observed. Exons 4-10 of TP53 showed no mutations or polymorphisms. The presence of HPV58 as single HPV infection in combination with a broad variety of direct and indirect markers of HPV transformation provides comprehensive evidence that this oropharyngeal SCC was driven by HPV58.
KW - Head and neck squamous cell carcinoma
KW - HPV antibody
KW - HPV carcinogenesis
KW - HPV E6 and E7
KW - HPV58
KW - p16§ssup§INK4a§esup§
KW - p53
KW - pRb
UR - http://www.scopus.com/inward/record.url?scp=84887276186&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887276186&partnerID=8YFLogxK
U2 - 10.1186/1743-422X-10-334
DO - 10.1186/1743-422X-10-334
M3 - Article
C2 - 24220072
AN - SCOPUS:84887276186
VL - 10
JO - Virology Journal
JF - Virology Journal
SN - 1743-422X
M1 - 334
ER -