Evidence that CHP100 neuroblastoma cell death induced by N-methyl-d-aspartate involves l-arginine-nitric oxide pathway activation

M. Tiziana Corasaniti, R. Laura Tartaglia, Gennaro Melino, Giuseppe Nisticò, Alessandro Finazzi-Agrò

Research output: Contribution to journalArticlepeer-review

Abstract

Evidence suggests that nitric oxide (NO) may mediate, at least in part, excitotoxic effects of excessive N-methyl-d-aspartate (NMDA) receptor activation both in vivo and in vitro. In the present experiments, NMDA-induced excitotoxicity has been studied in CHP100 neuroblastoma cell cultures. Application of NMDA (0.25-1.5 mM) produced concentration-dependent cell death. These effects were antagonized by co-application of dizocilpine (MK801), a selective and non-competitive NMDA receptor complex antagonist. Protection from NMDA-induced lethal effects was also afforded by Nω-nitro-l-arginine methyl ester, a potent NO-synthase inhibitor, and by hemoglobin, a NO-trapping agent. In addition, substitution of l-arginine, normally present in the exposure solution with its d-isomer, abolished the cell death induced by the excitotoxin. In conclusion, the present experiments support the suggestion that excitotoxic effects induced by NMDA receptor stimulation involve l-arginine-NO pathway activation.

Original languageEnglish
Pages (from-to)221-223
Number of pages3
JournalNeuroscience Letters
Volume147
Issue number2
DOIs
Publication statusPublished - Dec 7 1992

Keywords

  • CHP100 neuroblastoma
  • Dizocilpine
  • N-Methyl-d-aspartate
  • N-Nitro-l-arginine methyl ester
  • Nitric oxide

ASJC Scopus subject areas

  • Neuroscience(all)

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