Evidence that increases of mitochondrial immunoreactive IL-1β by HIV-1 gp120 implicate in situ cleavage of pro-IL-1β in the neocortex of rat

M. Tiziana Corasaniti, Paola Turano, Anna Bilotta, Walter Malorni, A. Rita Stringaro, Robert Nisticò, Alessandro Finazzi-Agró, Giacinto Bagetta

Research output: Contribution to journalArticle

Abstract

Immunoelectron microscopy analysis of brain tissue sections and rat-specific sandwich ELISA allowed the localization of interleukin-1β (IL-1β) immunoreactivity in the mitochondria and cytosol of neocortical tissue preparations from the brain of naive, untreated, rats and rats receiving a single daily injection into one lateral cerebral ventricle (i.c.v.) of bovine serum albumin (BSA; 100 ng/day) for seven consecutive days. Interestingly, seven days i.c.v. treatment with the HIV-1 coat protein gp120 (100 ng/day) enhances IL-1β immunoreactivity in the cellular fractions studied. Elevation of mitochondrial immunoreactive IL-1β levels seems to originate from the conversion operated by the interleukin converting enzyme (ICE) of mitochondrial pro-IL-1β; in fact, IL-1β increases reported in the ELISA experiments were paralleled by a decrease of the mitochondrial pro-IL-1β 31-kDa band in conjunction with enhanced expression of the p20 component of activated ICE. In conclusion, the present results demonstrate that gp120-enhanced neocortical expression of IL-1β originates, at least in part, from in situ cleavage of mitochondrial pro-IL-1β and suggest that this, together with the central role of the mitochondrion in the expression of programmed cell death, may be important for apoptosis induced by the viral coat protein in the brain of rats.

Original languageEnglish
Pages (from-to)611-618
Number of pages8
JournalJournal of Neurochemistry
Volume78
Issue number3
DOIs
Publication statusPublished - 2001

    Fingerprint

Keywords

  • Apoptosis
  • Human immunodeficiency virus type-1 glycoprotein 120
  • Interleukin converting enzyme
  • Interleukin-1β
  • Mitochondria
  • Pro-IL-1β

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Tiziana Corasaniti, M., Turano, P., Bilotta, A., Malorni, W., Rita Stringaro, A., Nisticò, R., Finazzi-Agró, A., & Bagetta, G. (2001). Evidence that increases of mitochondrial immunoreactive IL-1β by HIV-1 gp120 implicate in situ cleavage of pro-IL-1β in the neocortex of rat. Journal of Neurochemistry, 78(3), 611-618. https://doi.org/10.1046/j.1471-4159.2001.00441.x