TY - JOUR
T1 - Evidence that increases of mitochondrial immunoreactive IL-1β by HIV-1 gp120 implicate in situ cleavage of pro-IL-1β in the neocortex of rat
AU - Tiziana Corasaniti, M.
AU - Turano, Paola
AU - Bilotta, Anna
AU - Malorni, Walter
AU - Rita Stringaro, A.
AU - Nisticò, Robert
AU - Finazzi-Agró, Alessandro
AU - Bagetta, Giacinto
PY - 2001
Y1 - 2001
N2 - Immunoelectron microscopy analysis of brain tissue sections and rat-specific sandwich ELISA allowed the localization of interleukin-1β (IL-1β) immunoreactivity in the mitochondria and cytosol of neocortical tissue preparations from the brain of naive, untreated, rats and rats receiving a single daily injection into one lateral cerebral ventricle (i.c.v.) of bovine serum albumin (BSA; 100 ng/day) for seven consecutive days. Interestingly, seven days i.c.v. treatment with the HIV-1 coat protein gp120 (100 ng/day) enhances IL-1β immunoreactivity in the cellular fractions studied. Elevation of mitochondrial immunoreactive IL-1β levels seems to originate from the conversion operated by the interleukin converting enzyme (ICE) of mitochondrial pro-IL-1β; in fact, IL-1β increases reported in the ELISA experiments were paralleled by a decrease of the mitochondrial pro-IL-1β 31-kDa band in conjunction with enhanced expression of the p20 component of activated ICE. In conclusion, the present results demonstrate that gp120-enhanced neocortical expression of IL-1β originates, at least in part, from in situ cleavage of mitochondrial pro-IL-1β and suggest that this, together with the central role of the mitochondrion in the expression of programmed cell death, may be important for apoptosis induced by the viral coat protein in the brain of rats.
AB - Immunoelectron microscopy analysis of brain tissue sections and rat-specific sandwich ELISA allowed the localization of interleukin-1β (IL-1β) immunoreactivity in the mitochondria and cytosol of neocortical tissue preparations from the brain of naive, untreated, rats and rats receiving a single daily injection into one lateral cerebral ventricle (i.c.v.) of bovine serum albumin (BSA; 100 ng/day) for seven consecutive days. Interestingly, seven days i.c.v. treatment with the HIV-1 coat protein gp120 (100 ng/day) enhances IL-1β immunoreactivity in the cellular fractions studied. Elevation of mitochondrial immunoreactive IL-1β levels seems to originate from the conversion operated by the interleukin converting enzyme (ICE) of mitochondrial pro-IL-1β; in fact, IL-1β increases reported in the ELISA experiments were paralleled by a decrease of the mitochondrial pro-IL-1β 31-kDa band in conjunction with enhanced expression of the p20 component of activated ICE. In conclusion, the present results demonstrate that gp120-enhanced neocortical expression of IL-1β originates, at least in part, from in situ cleavage of mitochondrial pro-IL-1β and suggest that this, together with the central role of the mitochondrion in the expression of programmed cell death, may be important for apoptosis induced by the viral coat protein in the brain of rats.
KW - Apoptosis
KW - Human immunodeficiency virus type-1 glycoprotein 120
KW - Interleukin converting enzyme
KW - Interleukin-1β
KW - Mitochondria
KW - Pro-IL-1β
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U2 - 10.1046/j.1471-4159.2001.00441.x
DO - 10.1046/j.1471-4159.2001.00441.x
M3 - Article
C2 - 11483664
AN - SCOPUS:0034899840
VL - 78
SP - 611
EP - 618
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 3
ER -