Evidence that the KIR2DS5 gene codes for a surface receptor triggering natural killer cell function

Mariella Della Chiesa, Elisa Romeo, Michela Falco, Mirna Balsamo, Raffaella Augugliaro, Lorenzo Moretta, Cristina Bottino, Alessandro Moretta, Massimo Vitale

Research output: Contribution to journalArticle

Abstract

In this study, after immunization with NK cells from aKIR2DS5+ donor and screening on cell transfectants expressing different members of the killer immunoglobulin-like receptor (KIR) family, we generated a mAb, DF200, reacting with several KIR2D receptors including KIR2DL1/L2/L3, KIR2DS1/S2 and KIR2DS5. By the analysis of peripheral blood NK cells and in vitro derived NK cell clones, we have demonstrated for the first time that KIR2DS5 is expressed at the cell surface in discrete subsets of NK cells and, after DF200 mAb-mediated engagement, can induce both cytotoxicity and cytokine release. Using co-transfection and co-immunoprecipitation, we found that KIR2DS5 associates with the DAP12 signaling polypeptide. Finally, soluble KIR2DS5-Fc fusion protein does not bind to cell transfectants expressing different HLA-C alleles, suggesting that, if KIR2DS5 does recognize HLA-C molecules, this may only occur in the presence of certain peptides.

Original languageEnglish
Pages (from-to)2284-2289
Number of pages6
JournalEuropean Journal of Immunology
Volume38
Issue number8
DOIs
Publication statusPublished - Aug 2008

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Keywords

  • Cytotoxicity
  • Human NK cells
  • Killer immunoglobulin-like receptors

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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