TY - JOUR
T1 - Evidence that vascular endothelial cells can induce the retraction of fibrin clots
AU - Barbieri, B.
AU - Balconi, G.
AU - Dejana, E.
AU - Donati, M. B.
PY - 1981
Y1 - 1981
N2 - Cultured endothelial cells (EC) from bovine aorta and umbilical vein induced retraction of a fibrin clot formed by addition of thrombin to cell-free plasma. Fibrin clot retractile (FCR) activity increased with time (1-24 hr) and with the number of cells in the system (1-4 x 10
6/ml, final concentration), and was inhibited at 22° or in the presence of Na
2-EDTA; moreover, no retraction occurred when batroxobin was used as a clotting agent instead of thrombin. FCR of EC thus showed many characteristics in common with platelet- and fibroblast-induced clot retraction. FCR activity of bovine EC increased with the number of subcultures, being very low in cells harvested from primary cultures. In contrast, human EC had high activity in primary cultures. Like fibroblasts, EC with a higher density in culture showed lower FCR, suggesting that confluency inhibits the cell contractile capacity. FCR could thus represent a simple in vitro test to further characterize the biology of EC and to evaluate their role in the development of fibrin thrombi.
AB - Cultured endothelial cells (EC) from bovine aorta and umbilical vein induced retraction of a fibrin clot formed by addition of thrombin to cell-free plasma. Fibrin clot retractile (FCR) activity increased with time (1-24 hr) and with the number of cells in the system (1-4 x 10
6/ml, final concentration), and was inhibited at 22° or in the presence of Na
2-EDTA; moreover, no retraction occurred when batroxobin was used as a clotting agent instead of thrombin. FCR of EC thus showed many characteristics in common with platelet- and fibroblast-induced clot retraction. FCR activity of bovine EC increased with the number of subcultures, being very low in cells harvested from primary cultures. In contrast, human EC had high activity in primary cultures. Like fibroblasts, EC with a higher density in culture showed lower FCR, suggesting that confluency inhibits the cell contractile capacity. FCR could thus represent a simple in vitro test to further characterize the biology of EC and to evaluate their role in the development of fibrin thrombi.
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M3 - Article
C2 - 7348306
AN - SCOPUS:0019782707
VL - 168
SP - 204
EP - 207
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 0037-9727
IS - 2
ER -