Evolution of CTX-M-type β-lactamases in isolates of Escherichia coli infecting hospital and community patients

Gioconda Brigante, Francesco Luzzaro, Mariagrazia Perilli, Gianluigi Lombardi, Alessandra Colì, Gian Maria Rossolini, Gianfranco Amicosante, Antonio Toniolo

Research output: Contribution to journalArticle

Abstract

Escherichia coli isolates collected at our Institution from 1999 to 2003 (n = 20,258) were studied to evaluate the production of CTX-M-type extended-spectrum β-lactamases (ESBL). Isolates suspected of producing CTX-M enzymes were analyzed by the double-disk synergy test, hybridization with specific probes, PCR and direct DNA sequencing. Overall, 53 ESBL-positive isolates were found to carry CTX-M-type genes (blaCTX-M-1, n = 51; blaCTX-M-15, n = 2). The isolation of CTX-M-positive strains increased from 1 per year (1999) to 26 per year (2003). The first isolate carrying the blaCTX-M-15 gene appeared in 2003 and was obtained from a patient previously treated with ceftazidime. CTX-M-positive isolates were characterized by multi-drug resistance and were obtained both from inpatients (n = 29) and outpatients (n = 24). Most patients were over 60-year-old (n = 45), had underlying chronic diseases (n = 32), and had been hospitalized more than once (n = 33). Strains were frequently isolated from the urinary tract, often after recurrent infections. Our study demonstrates that CTX-M-producing isolates are increasing among E. coli strains. Adequate laboratory detection may help in choosing appropriate treatment and in limiting the spread of this resistance trait.

Original languageEnglish
Pages (from-to)157-162
Number of pages6
JournalInternational Journal of Antimicrobial Agents
Volume25
Issue number2
DOIs
Publication statusPublished - Feb 2005

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Keywords

  • Cefotaximases
  • ESBL
  • Multi-drug resistance
  • Urinary tract infection

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Brigante, G., Luzzaro, F., Perilli, M., Lombardi, G., Colì, A., Rossolini, G. M., Amicosante, G., & Toniolo, A. (2005). Evolution of CTX-M-type β-lactamases in isolates of Escherichia coli infecting hospital and community patients. International Journal of Antimicrobial Agents, 25(2), 157-162. https://doi.org/10.1016/j.ijantimicag.2004.09.013