Evolution of the E2 region of hepatitis C virus in an infant infected by mother-to-infant transmission

Giovanni Sitia, Davide Cella, Maria Stella De Mitri, Roberto Novati, Caterina Uberti Foppa, Katarina Perackis, Cesare Bianchi, Adriano Lazzarin, Giulia Morsica

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To demonstrate vertical transmission of hepatitis C virus from an HCV infected woman and to assess the evolution of HCV quasispecies in the infant, the variable E2 region was analyzed in one mother-infant pair at birth and in serial samples from the infected baby. Sequence analysis of the E2 region obtained by means of direct sequencing of PCR products of mother-infant pair at birth, showed that the sequence of the dominant strain in the infant was related closely but not identical to that of her mother. The HCV population in mother-infant pair at birth and in serial samples of the infant was analyzed by polymerase-chain reaction-mediated Single Strand Conformational Polymorphism analysis (SSCP), which can distinguish DNA fragments of the same size as different electrophoretic migration of single stranded DNA. Single Strand Polymorphism analysis revealed that the infant was infected with two mutant genomes whereas the mother had a unique variant. The prevalent strain detected in the baby was not dominant in the mother at delivery and the pattern of quasispecies in the infant at birth was not the same as her mother, suggesting that the infant acquired the infection in utero. Changes in the dominant strain and evolution of the pattern of quasispecies in the infant from the 10th month of age were possibly due to the immune selection of escape mutants.

Original languageEnglish
Pages (from-to)476-481
Number of pages6
JournalJournal of Medical Virology
Volume64
Issue number4
DOIs
Publication statusPublished - 2001

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Hepacivirus
Mothers
Parturition
Polymerase Chain Reaction
Single-Stranded DNA
Sequence Analysis
Genome
DNA
Infection

Keywords

  • HCV
  • Quasispecies
  • Single strand conformational polymorphism

ASJC Scopus subject areas

  • Virology

Cite this

Evolution of the E2 region of hepatitis C virus in an infant infected by mother-to-infant transmission. / Sitia, Giovanni; Cella, Davide; De Mitri, Maria Stella; Novati, Roberto; Foppa, Caterina Uberti; Perackis, Katarina; Bianchi, Cesare; Lazzarin, Adriano; Morsica, Giulia.

In: Journal of Medical Virology, Vol. 64, No. 4, 2001, p. 476-481.

Research output: Contribution to journalArticle

Sitia, Giovanni ; Cella, Davide ; De Mitri, Maria Stella ; Novati, Roberto ; Foppa, Caterina Uberti ; Perackis, Katarina ; Bianchi, Cesare ; Lazzarin, Adriano ; Morsica, Giulia. / Evolution of the E2 region of hepatitis C virus in an infant infected by mother-to-infant transmission. In: Journal of Medical Virology. 2001 ; Vol. 64, No. 4. pp. 476-481.
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