Evolutionary analysis of SARS-CoV-2: how mutation of Non-Structural Protein 6 (NSP6) could affect viral autophagy

Domenico Benvenuto; Silvia Angeletti; Marta Giovanetti; Martina Bianchi; Stefano Pascarella; Roberto Cauda; Massimo Ciccozzi; Antonio Cassone

doi:10.1016/j.jinf.2020.03.058

Evolutionary analysis of SARS-CoV-2: how mutation of Non-Structural Protein 6 (NSP6) could affect viral autophagy

Domenico Benvenuto, Silvia Angeletti, Marta Giovanetti, Martina Bianchi, Stefano Pascarella, Roberto Cauda, Massimo Ciccozzi, Antonio Cassone

IRCCS Fondazione Policlinico Universitario A. Gemelli

Research output: Contribution to journal › Article › peer-review

Abstract

Background: SARS-CoV-2 is a new coronavirus that has spread globally, infecting more than 150000 people, and being declared pandemic by the WHO. We provide here bio-informatic, evolutionary analysis of 351 available sequences of its genome with the aim of mapping genome structural variations and the patterns of selection. Methods: A Maximum likelihood tree has been built and selective pressure has been investigated in order to find any mutation developed during the SARS-CoV-2 epidemic that could potentially affect clinical evolution of the infection. Finding: We have found in more recent isolates the presence of two mutations affecting the Non-Structural Protein 6 (NSP6) and the Open Reding Frame10 (ORF 10) adjacent regions. Amino acidic change stability analysis suggests both mutations could confer lower stability of the protein structures. Interpretation: One of the two mutations, likely developed within the genome during virus spread, could affect virus intracellular survival. Genome follow-up of SARS-CoV-2 spread is urgently needed in order to identify mutations that could significantly modify virus pathogenicity.

Original language	English
Pages (from-to)	e24-e27
Journal	Journal of Infection
Volume	81
Issue number	1
DOIs	https://doi.org/10.1016/j.jinf.2020.03.058
Publication status	Published - Jul 2020

Keywords

Autophagy
Bio-informatic
Coronavirus
COVID-19
Molecular evolution
SARS-CoV-2

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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Evolutionary analysis of SARS-CoV-2 : how mutation of Non-Structural Protein 6 (NSP6) could affect viral autophagy. / Benvenuto, Domenico; Angeletti, Silvia; Giovanetti, Marta; Bianchi, Martina; Pascarella, Stefano; Cauda, Roberto; Ciccozzi, Massimo; Cassone, Antonio.

In: Journal of Infection, Vol. 81, No. 1, 07.2020, p. e24-e27.

Research output: Contribution to journal › Article › peer-review

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AU - Bianchi, Martina

AU - Pascarella, Stefano

AU - Cauda, Roberto

AU - Ciccozzi, Massimo

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