Evolving serodiagnostics by rationally designed peptide arrays: The Burkholderia paradigm in Cystic Fibrosis

Claudio Peri, Alessandro Gori, Paola Gagni, Laura Sola, Daniela Girelli, Samantha Sottotetti, Lisa Cariani, Marcella Chiari, Marina Cretich, Giorgio Colombo

Research output: Contribution to journalArticlepeer-review

Abstract

Efficient diagnosis of emerging and novel bacterial infections is fundamental to guide decisions on therapeutic treatments. Here, we engineered a novel rational strategy to design peptide microarray platforms, which combines structural and genomic analyses to predict the binding interfaces between diverse protein antigens and antibodies against Burkholderia cepacia complex infections present in the sera of Cystic Fibrosis (CF) patients. The predicted binding interfaces on the antigens are synthesized in the form of isolated peptides and chemically optimized for controlled orientation on the surface. Our platform displays multiple Burkholderia-related epitopes and is shown to diagnose infected individuals even in presence of superinfections caused by other prevalent CF pathogens, with limited cost and time requirements. Moreover, our data point out that the specific patterns determined by combined probe responses might provide a characterization of Burkholderia infections even at the subtype level (genomovars). The method is general and immediately applicable to other bacteria.

Original languageEnglish
Article number32873
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Sep 12 2016

ASJC Scopus subject areas

  • General

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