Ewing’s sarcoma: An analysis of miRNA expression profiles and target genes in paraffin-embedded primary tumor tissue

Antonina Parafioriti, Caterina Bason, Elisabetta Armiraglio, Lucia Calciano, Primo Andrea Daolio, Martina Berardocco, Andrea di Bernardo, Alessia Colosimo, Roberto Luksch, Anna C. Berardi

Research output: Contribution to journalArticlepeer-review

Abstract

The molecular mechanism responsible for Ewing’s Sarcoma (ES) remains largely unknown. MicroRNAs (miRNAs), a class of small non-coding RNAs able to regulate gene expression, are deregulated in tumors and may serve as a tool for diagnosis and prediction. However, the status of miRNAs in ES has not yet been thoroughly investigated. This study compared global miRNAs expression in paraffin-embedded tumor tissue samples from 20 ES patients, affected by primary untreated tumors, with miRNAs expressed in normal human mesenchymal stromal cells (MSCs) by microarray analysis. A miRTarBase database was used to identify the predicted target genes for differentially expressed miRNAs. The miRNAs microarray analysis revealed distinct patterns of miRNAs expression between ES samples and normal MSCs. 58 of the 954 analyzed miRNAs were significantly differentially expressed in ES samples compared to MSCs. Moreover, the qRT-PCR analysis carried out on three selected miRNAs showed that miR-181b, miR-1915 and miR-1275 were significantly aberrantly regulated, confirming the microarray results. Bio-database analysis identified BCL-2 as a bona fide target gene of the miR-21, miR-181a, miR-181b, miR-29a, miR-29b, miR-497, miR-195, miR-let-7a, miR-34a and miR-1915. Using paraffin-embedded tissues from ES patients, this study has identified several potential target miRNAs and one gene that might be considered a novel critical biomarker for ES pathogenesis.

Original languageEnglish
Article number656
JournalInternational Journal of Molecular Sciences
Volume17
Issue number5
DOIs
Publication statusPublished - May 1 2016

Keywords

  • Ewing’s Sarcoma
  • Human mesenchymal stem cells
  • MicroRNAs
  • MiRTarBase database

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications

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