Ex vivo behaviour of human bone tumor endothelial cells

Teresa Infante, Elena Cesario, Michele Gallo, Flavio Fazioli, Annarosaria De Chiara, Cristina Tutucci, Gaetano Apice, Filomena de Nigris

Research output: Contribution to journalArticle

Abstract

Cooperation between endothelial cells and bone in bone remodelling is well established. In contrast, bone microvasculature supporting the growth of primary tumors and metastasis is poorly understood. Several antiangiogenic agents have recently been undergoing trials, although an extensive body of clinical data and experimental research have proved that angiogenic pathways differ in each tumor type and stage. Here, for the first time, we characterize at the molecular and functional level tumor endothelial cells from human bone sarcomas at different stages of disease and with different histotypes. We selected a CD31 + subpopulation from biopsies that displayed the capability to grow as adherent cell lines without vascular endothelial growth factor (VEGF). Our findings show the existence in human primary bone sarcomas of highly proliferative endothelial cells expressing CD31, CD44, CD105, CD146 and CD90 markers. These cells are committed to develop capillary-like structures and colony formation units, and to produce nitric oxide. We believe that a better understanding of tumor vasculature could be a valid tool for the design of an efficacious antiangiogenic therapy as adjuvant treatment of sarcomas.

Original languageEnglish
Pages (from-to)404-417
Number of pages14
JournalCancers
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 2013

Keywords

  • Angiogenesis
  • Endothelial cells
  • Sarcomas
  • Tumor microenvironment

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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