Abstract
Background/Aims: Members of the melanoma antigen encoding gene family are expressed in tumors of different histological types but not in normal tissue. For this reason, they are attractive targets for cancer immunotherapy. Methods: In the present study, we analyzed the expression of MAGE-1 and -3 genes in the hepatocellular carcinoma (HCC) tissue as well as frequency, phenotype and function of circulating and tumor infiltrating CD8 + cells specific for HLA-A1 and -A2 restricted epitopes of MAGE-1 and -3. Results: Our study shows for the first time the presence of MAGE/tetramer + CD8 cells in the tumor tissue of patients with HCC. These cells are able to recognize the MAGE-1 sequence 161-169 and the MAGE-3 sequence 271-279. In a patient with a particularly high frequency of MAGE-1 sequence 161-169-specific T cells, phenotypic and functional analysis was performed showing a phenotype of recently-primed CD8 cells (CD28 + CD27 + CD45RA - CCR7). Conclusions: The observation of a spontaneous in vivo priming of a MAGE-specific T cell response in patients with HCC and the high frequency of MAGE antigens expression in this tumor, makes this antigen a potential candidate for a MAGE-specific immunotherapy in hepatocellular carcinoma.
| Original language | English |
|---|---|
| Pages (from-to) | 102-109 |
| Number of pages | 8 |
| Journal | Journal of Hepatology |
| Volume | 40 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2004 |
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Keywords
- Hepatocellular carcinoma
- Immunotherapy
- MAGE-3
- Melanoma antigen encoding gene-1
- Tumor immunity
ASJC Scopus subject areas
- Gastroenterology
Cite this
Ex vivo characterization of tumor-derived melanoma antigen encoding gene-specific CD8 + cells in patients with hepatocellular carcinoma. / Zerbini, Alessandro; Pilli, Massimo; Soliani, Paolo; Ziegler, Stefanie; Pelosi, Guido; Orlandini, Alessandra; Cavallo, Cristina; Uggeri, Jacopo; Scandroglio, Renato; Crafa, Pellegrino; Spagnoli, Giulio C.; Ferrari, Carlo; Missale, Gabriele.
In: Journal of Hepatology, Vol. 40, No. 1, 01.2004, p. 102-109.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ex vivo characterization of tumor-derived melanoma antigen encoding gene-specific CD8 + cells in patients with hepatocellular carcinoma
AU - Zerbini, Alessandro
AU - Pilli, Massimo
AU - Soliani, Paolo
AU - Ziegler, Stefanie
AU - Pelosi, Guido
AU - Orlandini, Alessandra
AU - Cavallo, Cristina
AU - Uggeri, Jacopo
AU - Scandroglio, Renato
AU - Crafa, Pellegrino
AU - Spagnoli, Giulio C.
AU - Ferrari, Carlo
AU - Missale, Gabriele
PY - 2004/1
Y1 - 2004/1
N2 - Background/Aims: Members of the melanoma antigen encoding gene family are expressed in tumors of different histological types but not in normal tissue. For this reason, they are attractive targets for cancer immunotherapy. Methods: In the present study, we analyzed the expression of MAGE-1 and -3 genes in the hepatocellular carcinoma (HCC) tissue as well as frequency, phenotype and function of circulating and tumor infiltrating CD8 + cells specific for HLA-A1 and -A2 restricted epitopes of MAGE-1 and -3. Results: Our study shows for the first time the presence of MAGE/tetramer + CD8 cells in the tumor tissue of patients with HCC. These cells are able to recognize the MAGE-1 sequence 161-169 and the MAGE-3 sequence 271-279. In a patient with a particularly high frequency of MAGE-1 sequence 161-169-specific T cells, phenotypic and functional analysis was performed showing a phenotype of recently-primed CD8 cells (CD28 + CD27 + CD45RA - CCR7). Conclusions: The observation of a spontaneous in vivo priming of a MAGE-specific T cell response in patients with HCC and the high frequency of MAGE antigens expression in this tumor, makes this antigen a potential candidate for a MAGE-specific immunotherapy in hepatocellular carcinoma.
AB - Background/Aims: Members of the melanoma antigen encoding gene family are expressed in tumors of different histological types but not in normal tissue. For this reason, they are attractive targets for cancer immunotherapy. Methods: In the present study, we analyzed the expression of MAGE-1 and -3 genes in the hepatocellular carcinoma (HCC) tissue as well as frequency, phenotype and function of circulating and tumor infiltrating CD8 + cells specific for HLA-A1 and -A2 restricted epitopes of MAGE-1 and -3. Results: Our study shows for the first time the presence of MAGE/tetramer + CD8 cells in the tumor tissue of patients with HCC. These cells are able to recognize the MAGE-1 sequence 161-169 and the MAGE-3 sequence 271-279. In a patient with a particularly high frequency of MAGE-1 sequence 161-169-specific T cells, phenotypic and functional analysis was performed showing a phenotype of recently-primed CD8 cells (CD28 + CD27 + CD45RA - CCR7). Conclusions: The observation of a spontaneous in vivo priming of a MAGE-specific T cell response in patients with HCC and the high frequency of MAGE antigens expression in this tumor, makes this antigen a potential candidate for a MAGE-specific immunotherapy in hepatocellular carcinoma.
KW - Hepatocellular carcinoma
KW - Immunotherapy
KW - MAGE-3
KW - Melanoma antigen encoding gene-1
KW - Tumor immunity
UR - http://www.scopus.com/inward/record.url?scp=0348103708&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0348103708&partnerID=8YFLogxK
U2 - 10.1016/S0168-8278(03)00484-7
DO - 10.1016/S0168-8278(03)00484-7
M3 - Article
C2 - 14672620
AN - SCOPUS:0348103708
VL - 40
SP - 102
EP - 109
JO - Journal of Hepatology
JF - Journal of Hepatology
SN - 0168-8278
IS - 1
ER -