Ex vivo evaluation of PPD-specific IFN-γ or IL-5 secreting cells in the peripheral blood and lungs of patients with tuberculosis

M. Morosini, F. Meloni, M. Uccelli, A. Marone Bianco, N. Solari, Anna Maria Fietta

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVES: To evaluate ex vivo purified protein derivative (PPD) specific Th1- and Th2-type functional responses in human tuberculosis (TB). DESIGN: IFN-γ and IL-5 secreting cells were measured by a computer-assisted ELISPOT assay in the peripheral blood of patients with pulmonary TB, in patients with other respiratory diseases (control patients) and in tuberculin skin test negative or positive healthy controls. Moreover, the number of IFN-γ or IL-5 spots was assessed in the bronchoalveolar lavage (BAL) cells of five patients with advanced TB and lung inflammation. RESULTS: The frequency of PPD-specific IFN-γ secreting cells in TB patients was higher than in control patients and healthy subjects. Although the number of PPD-specific IL-5 spots was low, a trend towards a higher frequency was observed in the peripheral blood of TB patients. Patients with advanced TB and lung inflammation had an increased number of both PPD-specific IFN-γ and IL-5 spots in BAL as compared to that in peripheral blood, but the IFN-γ/IL-5 ratio was about two-fold lower. CONCLUSIONS: In human TB, the host response in the periphery is driven by a specific Th1-type cytokine response, whereas in the lungs of patients with advanced disease and lung inflammation, polarisation towards a Th2-like bias is observed.

Original languageEnglish
Pages (from-to)753-759
Number of pages7
JournalInternational Journal of Tuberculosis and Lung Disease
Volume9
Issue number7
Publication statusPublished - Jul 2005

Keywords

  • BAL
  • Peripheral blood
  • PPD-specific IFN-γ spots
  • PPD-specific IL-5 spots
  • Tuberculosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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