Ex vivo expansion of mesenchymal stromal cells

Maria Ester Bernardo, Angela Maria Cometa, Daria Pagliara, Luciana Vinti, Francesca Rossi, Rosaria Cristantielli, Giuseppe Palumbo, Franco Locatelli

Research output: Contribution to journalArticlepeer-review


Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical application. Despite being efficacious, the use of fetal calf serum for MSC ex vivo expansion for clinical purposes raises concerns related to immunization and transmission of zoonoses; the standardization of expansion methods, possibly devoid of animal components, such as those based on platelet lysate, are discussed in this paper. Moreover, this review focuses on the search of novel markers for the prospective identification/isolation of MSCs and on the potential risks connected with ex vivo expansion of MSCs, in particular that of their malignant transformation. Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed.

Original languageEnglish
Pages (from-to)73-81
Number of pages9
JournalBest Practice and Research: Clinical Haematology
Issue number1
Publication statusPublished - Mar 2011


  • cellular therapy
  • ex vivo expansion
  • fetal calf serum
  • malignant transformation
  • mesenchymal stromal cells
  • platelet lysate

ASJC Scopus subject areas

  • Oncology
  • Clinical Biochemistry


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