Ex vivo gene transfer for improvement of transplanted pancreatic islet viability and function

S. Van Linthout, P. Madeddu

Research output: Contribution to journalArticlepeer-review

Abstract

Human pancreatic islet transplantation has recently been shown to be successful in replacing pancreatic endocrine function into type I diabetic recipients. A major drawback, however, is the high amount of pancreatic β cells required to render one single patient insulin-independent. Given the shortage of human β cell donors, the majority of type 1 diabetic patients remain excluded from this therapeutic option. High number of islets are requested since substantial islet cell death and dysfunction occur within the first few hours and days after islet transplantation. Impaired vascularization of the engraft, the non-specific inflammatory reaction at the site of transplantation, together with the presence of active or memory autoimmune responses to islet autoantigens and allogeneic recognition contribute to apoptosis of β cells and subsequent early graft function loss. This review will focus on ex vivo engineering of the islet graft by gene transfer to improve islet engraftment. An overview of currently used gene transfer techniques will be given and their potential will be discussed.

Original languageEnglish
Pages (from-to)2927-2940
Number of pages14
JournalCurrent Pharmaceutical Design
Volume11
Issue number22
DOIs
Publication statusPublished - 2005

Keywords

  • Angiogenesis
  • Diabetes
  • Engraftment
  • Ex Vivo gene transfer
  • Islets

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

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