Excitatory and inhibitory urinary bladder reflexes induced by stimulation of cervicovaginal capsaicin-sensitive sensory fibers in rats

Manuela Tramontana, Sandro Giuliani, Claudio Valenti, Cecilia Cialdai, Massimo Lazzeri, Damiano Turini, Carlo Alberto Maggi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have investigated the effect of intravaginal application of capsaicin on micturition reflex in female rats. Urinary bladder contractility was measured by transurethral pressure recording at isovolumetric and subthreshold conditions in anaesthetized rats. The intravaginal application of capsaicin (15 μg/50 μl rat) induced reproducible bladder phasic contractions, without desensitization upon repeated applications, that were blocked by intravenous atropine (1 mg/kg) or hexamethonium (5 mg/kg) and prevented by removal of paracervical ganglia or systemic capsaicin pretreatment (125 mg/kg, s.c.). The inhibition of sympathetic transmission by guanethidine (30 mg/kg, s.c.) produced significant increase of the bladder reflex contractions activated by intravaginal capsaicin. Intravenous administration of the TRPV1 antagonist, capsazepine (3 mg/kg), significantly reduced the excitatory reflex response to capsaicin. Intravaginal administration of capsaicin (15 μg/50 μl), during distension-induced reflex bladder contractions, produced a transient block of reflexes, unaffected by guanethidine pretreatment. In conclusion, the stimulation of capsaicin-sensitive sensory nerve endings in the rat cervix-vagina induced a dual excitatory or inhibitory bladder response in anaesthetized female rats depending on the degree of bladder distension.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume379
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Capsaicin
Reflex
Urinary Bladder
Guanethidine
Intravaginal Administration
Hexamethonium
Urination
Vagina
Sensory Receptor Cells
Atropine
Cervix Uteri
Ganglia
Intravenous Administration
Pressure

Keywords

  • Capsaicin
  • Cervicovesical reflex
  • Intravaginal
  • Micturition reflex
  • Sensory fibers
  • TRPV1

ASJC Scopus subject areas

  • Pharmacology

Cite this

Excitatory and inhibitory urinary bladder reflexes induced by stimulation of cervicovaginal capsaicin-sensitive sensory fibers in rats. / Tramontana, Manuela; Giuliani, Sandro; Valenti, Claudio; Cialdai, Cecilia; Lazzeri, Massimo; Turini, Damiano; Maggi, Carlo Alberto.

In: Naunyn-Schmiedeberg's Archives of Pharmacology, Vol. 379, No. 2, 02.2009, p. 107-114.

Research output: Contribution to journalArticle

Tramontana, Manuela ; Giuliani, Sandro ; Valenti, Claudio ; Cialdai, Cecilia ; Lazzeri, Massimo ; Turini, Damiano ; Maggi, Carlo Alberto. / Excitatory and inhibitory urinary bladder reflexes induced by stimulation of cervicovaginal capsaicin-sensitive sensory fibers in rats. In: Naunyn-Schmiedeberg's Archives of Pharmacology. 2009 ; Vol. 379, No. 2. pp. 107-114.
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