Excitotoxicity-related endocytosis in cortical neurons

A. Vaslin, J. Puyal, T. Borsello, P. G H Clarke

Research output: Contribution to journalArticle

Abstract

Recent studies showed that endocytosis is enhanced in neurons exposed to an excitototoxic stimulus. We here confirm and analyze this new phenomenon using dissociated cortical neuronal cultures. NMDA-induced uptake (FITC-dextran or FITC or horseradish peroxidase) occurs in these cultures and is due to endocytosis, not to cell entry through damaged membranes; it requires an excitotoxic dose of NMDA and is dependent on extracellular calcium, but occurs early, while the neuron is still intact and viable. It involves two components, NMDA-induced and constitutive, with different characteristics. Neither component involves specific binding of the endocytosed molecules to a saturable receptor. Strikingly, molecules internalized by the NMDA-induced component are targeted to neuronal nuclei. This component, but not the constitutive one, is blocked by a c-Jun N-terminal protein kinase inhibitor. In conclusion, an excitotoxic dose of NMDA triggers c-Jun N-terminal protein kinase-dependent endocytosis in cortical neuronal cultures, providing an in vitro model of the excitotoxicity-induced endocytosis reported in intact tissues.

Original languageEnglish
Pages (from-to)789-800
Number of pages12
JournalJournal of Neurochemistry
Volume102
Issue number3
DOIs
Publication statusPublished - Aug 2007

Fingerprint

N-Methylaspartate
Endocytosis
Neurons
JNK Mitogen-Activated Protein Kinases
Molecules
Fluorescein-5-isothiocyanate
Horseradish Peroxidase
Protein Kinase Inhibitors
Protein Kinases
Tissue
Calcium
Membranes
Proteins

Keywords

  • c-Jun N-terminal kinase
  • D-JNKI1
  • Dextran
  • Neurons
  • NMDA
  • Primary culture

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Excitotoxicity-related endocytosis in cortical neurons. / Vaslin, A.; Puyal, J.; Borsello, T.; Clarke, P. G H.

In: Journal of Neurochemistry, Vol. 102, No. 3, 08.2007, p. 789-800.

Research output: Contribution to journalArticle

Vaslin, A. ; Puyal, J. ; Borsello, T. ; Clarke, P. G H. / Excitotoxicity-related endocytosis in cortical neurons. In: Journal of Neurochemistry. 2007 ; Vol. 102, No. 3. pp. 789-800.
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