Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries

Dario Leosco, Guido Iaccarino, Ersilia Cipolletta, Domenico De Santis, Eliana Pisani, Valentina Trimarco, Nicola Ferrara, Pasquale Abete, Daniela Sorriento, Franco Rengo, Bruno Trimarco

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Aging is associated with alterations in β-adrenergic receptor (β-AR) signaling and reduction in cardiovascular responses to β-AR stimulation. Because exercise can attenuate age-related impairment in myocardial β-AR signaling and function, we tested whether training could also exert favorable effects on vascular β-AR responses. We evaluated common carotid artery responsiveness in isolated vessel ring preparations from 8 aged male Wistar-Kyoto (WKY) rats trained for 6 wk in a 5 days/wk swimming protocol, 10 untrained agematched rats, and 10 young WKY rats. Vessels were preconstricted with phenylephrine (10-6 M), and vasodilation was assessed in response to the β-AR agonist isoproterenol (10-10-3 × 10-8 M), the α2-AR agonist UK-14304 (10-9-10-6 M), the muscarinic receptor agonist ACh (10-9-10-6 M), and nitroprusside (10-8-10-5 M). β-AR density and cytoplasmic β-AR kinase (β-ARK) activity were tested on pooled carotid arteries. β-ARK expression was assessed in two endothelial cell lines from bovine aorta and aorta isolated from a 12-wk WKY rat. β-AR, α2-AR, and muscarinic responses, but not that to nitroprusside, were depressed in untrained aged vs. young animals. Exercise training restored β-AR and muscarinic responses but did not affect vasodilation induced by UK-14304 and nitroprusside. Aged carotid arteries showed reduced β-AR number and increased β-ARK activity. Training counterbalanced these phenomena and restored β-AR density and β-ARK activity to levels observed in young rat carotids. Our data indicate that age impairs β-AR vasorelaxation in rat carotid arteries through β-AR downregulation and desensitization. Exercise restores this response and reverts age-related modification in β-ARs and β-ARK. Our data support an important role for β-ARK in vascular β-AR vasorelaxation.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume285
Issue number1 54-1
Publication statusPublished - Jul 1 2003

Fingerprint

Carotid Arteries
Vasodilation
Adrenergic Agents
Inbred WKY Rats
Nitroprusside
Cholinergic Agents
Blood Vessels
Aorta
Muscarinic Agonists
Common Carotid Artery
Phenylephrine
Muscarinic Receptors
Isoproterenol
Adrenergic Receptors
Phosphotransferases
Down-Regulation
Endothelial Cells
Exercise
Cell Line
Brimonidine Tartrate

Keywords

  • Aging
  • Beta-adrenergic receptor
  • Exercise

ASJC Scopus subject areas

  • Physiology

Cite this

Leosco, D., Iaccarino, G., Cipolletta, E., De Santis, D., Pisani, E., Trimarco, V., ... Trimarco, B. (2003). Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries. American Journal of Physiology - Heart and Circulatory Physiology, 285(1 54-1).

Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries. / Leosco, Dario; Iaccarino, Guido; Cipolletta, Ersilia; De Santis, Domenico; Pisani, Eliana; Trimarco, Valentina; Ferrara, Nicola; Abete, Pasquale; Sorriento, Daniela; Rengo, Franco; Trimarco, Bruno.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 285, No. 1 54-1, 01.07.2003.

Research output: Contribution to journalArticle

Leosco, D, Iaccarino, G, Cipolletta, E, De Santis, D, Pisani, E, Trimarco, V, Ferrara, N, Abete, P, Sorriento, D, Rengo, F & Trimarco, B 2003, 'Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries', American Journal of Physiology - Heart and Circulatory Physiology, vol. 285, no. 1 54-1.
Leosco D, Iaccarino G, Cipolletta E, De Santis D, Pisani E, Trimarco V et al. Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries. American Journal of Physiology - Heart and Circulatory Physiology. 2003 Jul 1;285(1 54-1).
Leosco, Dario ; Iaccarino, Guido ; Cipolletta, Ersilia ; De Santis, Domenico ; Pisani, Eliana ; Trimarco, Valentina ; Ferrara, Nicola ; Abete, Pasquale ; Sorriento, Daniela ; Rengo, Franco ; Trimarco, Bruno. / Exercise restores β-adrenergic vasorelaxation in aged rat carotid arteries. In: American Journal of Physiology - Heart and Circulatory Physiology. 2003 ; Vol. 285, No. 1 54-1.
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