Exercise training reduces serum capacity to induce endothelial cell death in patients with chronic heart failure

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Abstract

AimsPhysical training improves endothelial function and exercise capacity in patients with heart failure (HF). Serum from patients with cardiovascular diseases increases apoptosis of human endothelial cells suggesting the importance of humoral factors in the progression of the disease. We evaluated whether exercise training influences the apoptotic capacity of serum from patients with chronic HF (CHF).Methods and resultsThe study included 39 patients with HF (NYHA II) and 10 age-matched healthy controls. Patients were allocated to either a structured programme of exercise training (24 patients) or standard care (15 patients). Human umbilical vein endothelial cells (HUVECs) were incubated with a medium containing 20 serum obtained before and after either a 3-week exercise training programme or standard care. At baseline, serum from patients with CHF induced a higher degree of lactate dehydrogenase (LDH) release and apoptosis in HUVECs compared with healthy controls (43 ± 1.5 vs. 16 ± 1.1, P<0.001 and 67 ± 5.4 vs. 23 ± 5.8, P<0.001, respectively). Exercise training significantly increased performance in the 6 min walking test (34.7) and reduced the ability of serum to induce LDH release and apoptosis of HUVECs. The reduction of apoptosis after exercise training correlated with the improvement in functional capacity. The expression of the apoptosis markers Bax and Caspase-3 was significantly reduced in HUVECs exposed to serum collected after exercise training. Circulating tumour necrosis factor-alpha, matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinase-1 (TIMP-1) levels were significantly reduced by exercise training and the MMP-9/TIMP-1 ratio increased.ConclusionA short term in-hospital structured cardiovascular training programme reduces the ability of serum-derived factors to induce endothelial cell death in patients with CHF.

Original languageEnglish
Pages (from-to)642-650
Number of pages9
JournalEuropean Journal of Heart Failure
Volume13
Issue number6
DOIs
Publication statusPublished - Jun 2011

Fingerprint

Cell Death
Endothelial Cells
Heart Failure
Exercise
Human Umbilical Vein Endothelial Cells
Serum
Apoptosis
Tissue Inhibitor of Metalloproteinase-1
L-Lactate Dehydrogenase
Education
Matrix Metalloproteinase 1
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinases
Caspase 3
Walking
Disease Progression
Patient Care
Cardiovascular Diseases
Tumor Necrosis Factor-alpha

Keywords

  • Apoptosis
  • Endothelial cells
  • Endothelial function
  • Exercise training
  • Heart failure
  • Inflammation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

@article{5f19a2df4ab34d26b33a2eb21b00f07d,
title = "Exercise training reduces serum capacity to induce endothelial cell death in patients with chronic heart failure",
abstract = "AimsPhysical training improves endothelial function and exercise capacity in patients with heart failure (HF). Serum from patients with cardiovascular diseases increases apoptosis of human endothelial cells suggesting the importance of humoral factors in the progression of the disease. We evaluated whether exercise training influences the apoptotic capacity of serum from patients with chronic HF (CHF).Methods and resultsThe study included 39 patients with HF (NYHA II) and 10 age-matched healthy controls. Patients were allocated to either a structured programme of exercise training (24 patients) or standard care (15 patients). Human umbilical vein endothelial cells (HUVECs) were incubated with a medium containing 20 serum obtained before and after either a 3-week exercise training programme or standard care. At baseline, serum from patients with CHF induced a higher degree of lactate dehydrogenase (LDH) release and apoptosis in HUVECs compared with healthy controls (43 ± 1.5 vs. 16 ± 1.1, P<0.001 and 67 ± 5.4 vs. 23 ± 5.8, P<0.001, respectively). Exercise training significantly increased performance in the 6 min walking test (34.7) and reduced the ability of serum to induce LDH release and apoptosis of HUVECs. The reduction of apoptosis after exercise training correlated with the improvement in functional capacity. The expression of the apoptosis markers Bax and Caspase-3 was significantly reduced in HUVECs exposed to serum collected after exercise training. Circulating tumour necrosis factor-alpha, matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinase-1 (TIMP-1) levels were significantly reduced by exercise training and the MMP-9/TIMP-1 ratio increased.ConclusionA short term in-hospital structured cardiovascular training programme reduces the ability of serum-derived factors to induce endothelial cell death in patients with CHF.",
keywords = "Apoptosis, Endothelial cells, Endothelial function, Exercise training, Heart failure, Inflammation",
author = "Caterina Mammi and Sala, {Andrea La} and Maurizio Volterrani and Lucia Gatta and Antonella Antelmi and Alessandra Feraco and Giuseppe Caminiti and Giuseppe Marazzi and Cristiana Vitale and Massimiliano Caprio and Rosano, {Giuseppe Massimo Claudio}",
year = "2011",
month = "6",
doi = "10.1093/eurjhf/hfr026",
language = "English",
volume = "13",
pages = "642--650",
journal = "European Journal of Heart Failure",
issn = "1388-9842",
publisher = "John Wiley & Sons, Ltd",
number = "6",

}

TY - JOUR

T1 - Exercise training reduces serum capacity to induce endothelial cell death in patients with chronic heart failure

AU - Mammi, Caterina

AU - Sala, Andrea La

AU - Volterrani, Maurizio

AU - Gatta, Lucia

AU - Antelmi, Antonella

AU - Feraco, Alessandra

AU - Caminiti, Giuseppe

AU - Marazzi, Giuseppe

AU - Vitale, Cristiana

AU - Caprio, Massimiliano

AU - Rosano, Giuseppe Massimo Claudio

PY - 2011/6

Y1 - 2011/6

N2 - AimsPhysical training improves endothelial function and exercise capacity in patients with heart failure (HF). Serum from patients with cardiovascular diseases increases apoptosis of human endothelial cells suggesting the importance of humoral factors in the progression of the disease. We evaluated whether exercise training influences the apoptotic capacity of serum from patients with chronic HF (CHF).Methods and resultsThe study included 39 patients with HF (NYHA II) and 10 age-matched healthy controls. Patients were allocated to either a structured programme of exercise training (24 patients) or standard care (15 patients). Human umbilical vein endothelial cells (HUVECs) were incubated with a medium containing 20 serum obtained before and after either a 3-week exercise training programme or standard care. At baseline, serum from patients with CHF induced a higher degree of lactate dehydrogenase (LDH) release and apoptosis in HUVECs compared with healthy controls (43 ± 1.5 vs. 16 ± 1.1, P<0.001 and 67 ± 5.4 vs. 23 ± 5.8, P<0.001, respectively). Exercise training significantly increased performance in the 6 min walking test (34.7) and reduced the ability of serum to induce LDH release and apoptosis of HUVECs. The reduction of apoptosis after exercise training correlated with the improvement in functional capacity. The expression of the apoptosis markers Bax and Caspase-3 was significantly reduced in HUVECs exposed to serum collected after exercise training. Circulating tumour necrosis factor-alpha, matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinase-1 (TIMP-1) levels were significantly reduced by exercise training and the MMP-9/TIMP-1 ratio increased.ConclusionA short term in-hospital structured cardiovascular training programme reduces the ability of serum-derived factors to induce endothelial cell death in patients with CHF.

AB - AimsPhysical training improves endothelial function and exercise capacity in patients with heart failure (HF). Serum from patients with cardiovascular diseases increases apoptosis of human endothelial cells suggesting the importance of humoral factors in the progression of the disease. We evaluated whether exercise training influences the apoptotic capacity of serum from patients with chronic HF (CHF).Methods and resultsThe study included 39 patients with HF (NYHA II) and 10 age-matched healthy controls. Patients were allocated to either a structured programme of exercise training (24 patients) or standard care (15 patients). Human umbilical vein endothelial cells (HUVECs) were incubated with a medium containing 20 serum obtained before and after either a 3-week exercise training programme or standard care. At baseline, serum from patients with CHF induced a higher degree of lactate dehydrogenase (LDH) release and apoptosis in HUVECs compared with healthy controls (43 ± 1.5 vs. 16 ± 1.1, P<0.001 and 67 ± 5.4 vs. 23 ± 5.8, P<0.001, respectively). Exercise training significantly increased performance in the 6 min walking test (34.7) and reduced the ability of serum to induce LDH release and apoptosis of HUVECs. The reduction of apoptosis after exercise training correlated with the improvement in functional capacity. The expression of the apoptosis markers Bax and Caspase-3 was significantly reduced in HUVECs exposed to serum collected after exercise training. Circulating tumour necrosis factor-alpha, matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinase-1 (TIMP-1) levels were significantly reduced by exercise training and the MMP-9/TIMP-1 ratio increased.ConclusionA short term in-hospital structured cardiovascular training programme reduces the ability of serum-derived factors to induce endothelial cell death in patients with CHF.

KW - Apoptosis

KW - Endothelial cells

KW - Endothelial function

KW - Exercise training

KW - Heart failure

KW - Inflammation

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U2 - 10.1093/eurjhf/hfr026

DO - 10.1093/eurjhf/hfr026

M3 - Article

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AN - SCOPUS:79957794709

VL - 13

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JO - European Journal of Heart Failure

JF - European Journal of Heart Failure

SN - 1388-9842

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