Abstract
We measured 8-isoprostane, a biomarker of oxidative stress, and prostaglandin (PG) E2 in exhaled breath condensate in 36 stable and 14 unstable cystic fibrosis (CF) patients, and in 15 healthy age-matched controls. We studied the relationships of these eicosanoids with clinical, radiological, and systemic inflammatory parameters. Compared with controls [15.5 (11.5-17.0) pg/ml] exhaled 8-isoprostane was increased in stable CF patients [30.5 (25.3-36.0) pg/ml, P <0.001]. Unstable CF patients had higher exhaled 8-isoprostane levels [47.5 (44.0-50.0) pg/ml, P <0.001] than stable CF patients. Unlike PGE2, exhaled 8-isoprostane was negatively correlated with FEV1 (r = -0.67; P <0.0001; r = -0.63; P <0.02) and Shwachman score (r = -0.43, P = 0.012; r = -0.58, P = 0.031) and positively correlated with Chrispin-Norman score (r = 0.51, P <0.002; r = 0.56, P = 0.039) in stable and unstable CF patients, respectively. No correlation was observed with C-reactive protein. Compared with controls [41.0 (29.0-50.0) pg/ml], exhaled PGE2 was also elevated in stable [72.0 (64.3-81.8) pg/ml, P <0.001) and, to a greater extent, in unstable CF patients [83.0 (74.3-91.3) pg/ml, P <0.001). In patients with CF, exhaled 8-isoprostane and PGE2 could be a useful marker of disease severity.
| Original language | English |
|---|---|
| Pages (from-to) | 913-919 |
| Number of pages | 7 |
| Journal | Free Radical Biology and Medicine |
| Volume | 45 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Sep 15 2008 |
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Keywords
- 8-Isoprostane
- Oxidative stress
- Prostaglandin E
- Stable cystic fibrosis
- Unstable cystic fibrosis
ASJC Scopus subject areas
- Biochemistry
- Physiology (medical)
Cite this
Exhaled 8-isoprostane and prostaglandin E2 in patients with stable and unstable cystic fibrosis{star, open} . / Lucidi, Vincenzina; Ciabattoni, Giovanni; Bella, Sergio; Barnes, Peter J.; Montuschi, Paolo.
In: Free Radical Biology and Medicine, Vol. 45, No. 6, 15.09.2008, p. 913-919.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Exhaled 8-isoprostane and prostaglandin E2 in patients with stable and unstable cystic fibrosis{star, open}
AU - Lucidi, Vincenzina
AU - Ciabattoni, Giovanni
AU - Bella, Sergio
AU - Barnes, Peter J.
AU - Montuschi, Paolo
PY - 2008/9/15
Y1 - 2008/9/15
N2 - We measured 8-isoprostane, a biomarker of oxidative stress, and prostaglandin (PG) E2 in exhaled breath condensate in 36 stable and 14 unstable cystic fibrosis (CF) patients, and in 15 healthy age-matched controls. We studied the relationships of these eicosanoids with clinical, radiological, and systemic inflammatory parameters. Compared with controls [15.5 (11.5-17.0) pg/ml] exhaled 8-isoprostane was increased in stable CF patients [30.5 (25.3-36.0) pg/ml, P <0.001]. Unstable CF patients had higher exhaled 8-isoprostane levels [47.5 (44.0-50.0) pg/ml, P <0.001] than stable CF patients. Unlike PGE2, exhaled 8-isoprostane was negatively correlated with FEV1 (r = -0.67; P <0.0001; r = -0.63; P <0.02) and Shwachman score (r = -0.43, P = 0.012; r = -0.58, P = 0.031) and positively correlated with Chrispin-Norman score (r = 0.51, P <0.002; r = 0.56, P = 0.039) in stable and unstable CF patients, respectively. No correlation was observed with C-reactive protein. Compared with controls [41.0 (29.0-50.0) pg/ml], exhaled PGE2 was also elevated in stable [72.0 (64.3-81.8) pg/ml, P <0.001) and, to a greater extent, in unstable CF patients [83.0 (74.3-91.3) pg/ml, P <0.001). In patients with CF, exhaled 8-isoprostane and PGE2 could be a useful marker of disease severity.
AB - We measured 8-isoprostane, a biomarker of oxidative stress, and prostaglandin (PG) E2 in exhaled breath condensate in 36 stable and 14 unstable cystic fibrosis (CF) patients, and in 15 healthy age-matched controls. We studied the relationships of these eicosanoids with clinical, radiological, and systemic inflammatory parameters. Compared with controls [15.5 (11.5-17.0) pg/ml] exhaled 8-isoprostane was increased in stable CF patients [30.5 (25.3-36.0) pg/ml, P <0.001]. Unstable CF patients had higher exhaled 8-isoprostane levels [47.5 (44.0-50.0) pg/ml, P <0.001] than stable CF patients. Unlike PGE2, exhaled 8-isoprostane was negatively correlated with FEV1 (r = -0.67; P <0.0001; r = -0.63; P <0.02) and Shwachman score (r = -0.43, P = 0.012; r = -0.58, P = 0.031) and positively correlated with Chrispin-Norman score (r = 0.51, P <0.002; r = 0.56, P = 0.039) in stable and unstable CF patients, respectively. No correlation was observed with C-reactive protein. Compared with controls [41.0 (29.0-50.0) pg/ml], exhaled PGE2 was also elevated in stable [72.0 (64.3-81.8) pg/ml, P <0.001) and, to a greater extent, in unstable CF patients [83.0 (74.3-91.3) pg/ml, P <0.001). In patients with CF, exhaled 8-isoprostane and PGE2 could be a useful marker of disease severity.
KW - 8-Isoprostane
KW - Oxidative stress
KW - Prostaglandin E
KW - Stable cystic fibrosis
KW - Unstable cystic fibrosis
UR - http://www.scopus.com/inward/record.url?scp=54949110924&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=54949110924&partnerID=8YFLogxK
U2 - 10.1016/j.freeradbiomed.2008.06.026
DO - 10.1016/j.freeradbiomed.2008.06.026
M3 - Article
C2 - 18634869
AN - SCOPUS:54949110924
VL - 45
SP - 913
EP - 919
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
IS - 6
ER -