Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes

Domenico Ribatti, Paola Locci, Lorella Marinucci, Cinzia Lilli, Luisa Roncali, E. Becchetti

Research output: Contribution to journalArticle

Abstract

In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of 3H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalInternational journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation
Volume15
Issue number4
DOIs
Publication statusPublished - 1995

Keywords

  • Angiogenesis
  • Chorioallantoic membrane
  • Glycosaminoglycans
  • Heparin

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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