In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of 3H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.
|Number of pages||5|
|Journal||International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation|
|Publication status||Published - 1995|
- Chorioallantoic membrane
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine