Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes

Domenico Ribatti; Paola Locci; Lorella Marinucci; Cinzia Lilli; Luisa Roncali; E. Becchetti

doi:10.1159/000178973

Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes

Domenico Ribatti, Paola Locci, Lorella Marinucci, Cinzia Lilli, Luisa Roncali, E. Becchetti

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article › peer-review

Abstract

In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of ³H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.

Original language	English
Pages (from-to)	181-185
Number of pages	5
Journal	International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation
Volume	15
Issue number	4
DOIs	https://doi.org/10.1159/000178973
Publication status	Published - 1995

Keywords

Angiogenesis
Chorioallantoic membrane
Glycosaminoglycans
Heparin

ASJC Scopus subject areas

Physiology
Cardiology and Cardiovascular Medicine

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Ribatti, D., Locci, P., Marinucci, L., Lilli, C., Roncali, L., & Becchetti, E. (1995). Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes. International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation, 15(4), 181-185. https://doi.org/10.1159/000178973

Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane : Its possible role in the regulation of angiogenic processes. / Ribatti, Domenico; Locci, Paola; Marinucci, Lorella; Lilli, Cinzia; Roncali, Luisa; Becchetti, E.

In: International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation, Vol. 15, No. 4, 1995, p. 181-185.

Research output: Contribution to journal › Article › peer-review

Ribatti, D, Locci, P, Marinucci, L, Lilli, C, Roncali, L & Becchetti, E 1995, 'Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes', International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation, vol. 15, no. 4, pp. 181-185. https://doi.org/10.1159/000178973

Ribatti D, Locci P, Marinucci L, Lilli C, Roncali L, Becchetti E. Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes. International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation. 1995;15(4):181-185. https://doi.org/10.1159/000178973

Ribatti, Domenico ; Locci, Paola ; Marinucci, Lorella ; Lilli, Cinzia ; Roncali, Luisa ; Becchetti, E. / Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane : Its possible role in the regulation of angiogenic processes. In: International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation. 1995 ; Vol. 15, No. 4. pp. 181-185.

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abstract = "In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of 3H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.",

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AU - Marinucci, Lorella

AU - Lilli, Cinzia

AU - Roncali, Luisa

AU - Becchetti, E.

PY - 1995

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N2 - In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of 3H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.

AB - In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of 3H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.

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Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes

Abstract

Keywords

ASJC Scopus subject areas

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