Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes

Domenico Ribatti; Paola Locci; Lorella Marinucci; Cinzia Lilli; Luisa Roncali; E. Becchetti

doi:10.1159/000178973

Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes

Domenico Ribatti, Paola Locci, Lorella Marinucci, Cinzia Lilli, Luisa Roncali, E. Becchetti

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article › peer-review

Abstract

In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of ³H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.

Original language	English
Pages (from-to)	181-185
Number of pages	5
Journal	International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation
Volume	15
Issue number	4
DOIs	https://doi.org/10.1159/000178973
Publication status	Published - 1995

Keywords

Angiogenesis
Chorioallantoic membrane
Glycosaminoglycans
Heparin

ASJC Scopus subject areas

Physiology
Cardiology and Cardiovascular Medicine

Access to Document

10.1159/000178973

Cite this

Ribatti, D., Locci, P., Marinucci, L., Lilli, C., Roncali, L., & Becchetti, E. (1995). Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes. International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation, 15(4), 181-185. https://doi.org/10.1159/000178973

Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane : Its possible role in the regulation of angiogenic processes. / Ribatti, Domenico; Locci, Paola; Marinucci, Lorella et al.

In: International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation, Vol. 15, No. 4, 1995, p. 181-185.

Research output: Contribution to journal › Article › peer-review

Ribatti, D, Locci, P, Marinucci, L, Lilli, C, Roncali, L & Becchetti, E 1995, 'Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes', International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation, vol. 15, no. 4, pp. 181-185. https://doi.org/10.1159/000178973

Ribatti D, Locci P, Marinucci L, Lilli C, Roncali L, Becchetti E. Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes. International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation. 1995;15(4):181-185. https://doi.org/10.1159/000178973

Ribatti, Domenico ; Locci, Paola ; Marinucci, Lorella et al. / Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane : Its possible role in the regulation of angiogenic processes. In: International journal of microcirculation, clinical and experimental / sponsored by the European Society for Microcirculation. 1995 ; Vol. 15, No. 4. pp. 181-185.

@article{6599acc868a5485ab6ebcde98b38c304,

title = "Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes",

abstract = "In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of 3H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.",

keywords = "Angiogenesis, Chorioallantoic membrane, Glycosaminoglycans, Heparin",

author = "Domenico Ribatti and Paola Locci and Lorella Marinucci and Cinzia Lilli and Luisa Roncali and E. Becchetti",

year = "1995",

doi = "10.1159/000178973",

language = "English",

volume = "15",

pages = "181--185",

journal = "International Journal of Microcirculation-Clinical and Experimental",

issn = "0167-6865",

publisher = "S. Karger AG",

number = "4",

}

TY - JOUR

T1 - Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane

T2 - Its possible role in the regulation of angiogenic processes

AU - Ribatti, Domenico

AU - Locci, Paola

AU - Marinucci, Lorella

AU - Lilli, Cinzia

AU - Roncali, Luisa

AU - Becchetti, E.

PY - 1995

Y1 - 1995

N2 - In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of 3H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.

AB - In this study heparin (HE) was injected into the allantoic sac of chick embryo eggs at 5. 9, 14 days of incubation. 48 h after injection glycosaminoglyean (GAG) concentration was measured in the chorioallantoic membrane (CAM) in order to verify if HE-related CAM angiogenic activity previously demonstrated [Ribatti et al: Acta Anat 1987; 130: 257-263] might be correlated with changes in GAG concentration. The results showed that HE inoculation induced an increase of 3H-glucosamine incorporation into total GAGs in comparison to control CAMs. Furthermore, HE altered the balance between the GAG classes, and in particular it produced a significant increase in the accumulation of hyaluronic acid and heparan sulfate between 7 and 11 days of incubation in comparison to control CAMs.

KW - Angiogenesis

KW - Chorioallantoic membrane

KW - Glycosaminoglycans

KW - Heparin

UR - http://www.scopus.com/inward/record.url?scp=0029330019&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029330019&partnerID=8YFLogxK

U2 - 10.1159/000178973

DO - 10.1159/000178973

M3 - Article

C2 - 8847178

AN - SCOPUS:0029330019

VL - 15

SP - 181

EP - 185

JO - International Journal of Microcirculation-Clinical and Experimental

JF - International Journal of Microcirculation-Clinical and Experimental

SN - 0167-6865

IS - 4

ER -

Exogenous heparin induces an increase in glycosaminoglycans of the chick embryo chorioallantoic membrane: Its possible role in the regulation of angiogenic processes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this