Exogenous surfactant kinetics in infant respiratory distress syndrome

A novel method with stable isotopes

Mirka Torresin, L. J I Zimmermann, Paola E. Cogo, Paola Cavicchioli, Tamara Badon, Giuseppe Giordano, Franco Zacchello, P. J J Sauer, Virgilio P. Carnielli

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Little is known about surfactant metabolism in newborn infants, since radioactive isotopes cannot be used in humans. We describe here a new method for studying exogenous surfactant pharmacokinetics in vivo. We measured surfactant half-life, pool size, and turnover time in eight preterm infants (gestational age: 30 ± 2 wk; birth weight: 1,416 ± 202 g) who were mechanically ventilated because of infant respiratory distress syndrome. We administered two doses of 100 mg/kg each of a natural porcine surfactant with 13C-labeled dipalmitoylphosphatidylcholine as a tracer. The 13C enrichment of surfactant disaturated phosphatidylcholine (DSPC) was measured in serial tracheal aspirates by gas chromatography-mass spectrometry. The DSPC half-life was 34.2 ± 9.4 h (mean ± SD; range: 21.8 to 45.9 h). The apparent DSPC pool sizes were 5.8 ± 6.1 mg/kg (range: 0.1 to 17.0 mg/kg) and 17.3 ± 13.6 mg/kg (range: 3.3 to 41.0 mg/kg) at the time of the first and second surfactant doses, respectively. We present a novel and safe method that allows the tracing of exogenous surfactant phosphatidylcholine, the major lipid component of pulmonary surfactant, in infants who receive exogenous surfactant. This method could be a valuable tool for studying: (1) therapies that enhance lung/surfactant maturation; (2) the dosing and timing of surfactant therapy in different lung diseases; and (3) the comparison of different surfactant preparations.

Original languageEnglish
Pages (from-to)1584-1589
Number of pages6
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume161
Issue number5
Publication statusPublished - 2000

Fingerprint

Newborn Respiratory Distress Syndrome
Surface-Active Agents
Isotopes
Half-Life
Pulmonary Surfactants
1,2-Dipalmitoylphosphatidylcholine
Phosphatidylcholines
Birth Weight
Premature Infants
Radioisotopes
Gas Chromatography-Mass Spectrometry
Lung Diseases
Gestational Age
Swine
Pharmacokinetics

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Torresin, M., Zimmermann, L. J. I., Cogo, P. E., Cavicchioli, P., Badon, T., Giordano, G., ... Carnielli, V. P. (2000). Exogenous surfactant kinetics in infant respiratory distress syndrome: A novel method with stable isotopes. American Journal of Respiratory and Critical Care Medicine, 161(5), 1584-1589.

Exogenous surfactant kinetics in infant respiratory distress syndrome : A novel method with stable isotopes. / Torresin, Mirka; Zimmermann, L. J I; Cogo, Paola E.; Cavicchioli, Paola; Badon, Tamara; Giordano, Giuseppe; Zacchello, Franco; Sauer, P. J J; Carnielli, Virgilio P.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 161, No. 5, 2000, p. 1584-1589.

Research output: Contribution to journalArticle

Torresin, M, Zimmermann, LJI, Cogo, PE, Cavicchioli, P, Badon, T, Giordano, G, Zacchello, F, Sauer, PJJ & Carnielli, VP 2000, 'Exogenous surfactant kinetics in infant respiratory distress syndrome: A novel method with stable isotopes', American Journal of Respiratory and Critical Care Medicine, vol. 161, no. 5, pp. 1584-1589.
Torresin, Mirka ; Zimmermann, L. J I ; Cogo, Paola E. ; Cavicchioli, Paola ; Badon, Tamara ; Giordano, Giuseppe ; Zacchello, Franco ; Sauer, P. J J ; Carnielli, Virgilio P. / Exogenous surfactant kinetics in infant respiratory distress syndrome : A novel method with stable isotopes. In: American Journal of Respiratory and Critical Care Medicine. 2000 ; Vol. 161, No. 5. pp. 1584-1589.
@article{e4a3f790fcab4c5f81635a18b48d3db3,
title = "Exogenous surfactant kinetics in infant respiratory distress syndrome: A novel method with stable isotopes",
abstract = "Little is known about surfactant metabolism in newborn infants, since radioactive isotopes cannot be used in humans. We describe here a new method for studying exogenous surfactant pharmacokinetics in vivo. We measured surfactant half-life, pool size, and turnover time in eight preterm infants (gestational age: 30 ± 2 wk; birth weight: 1,416 ± 202 g) who were mechanically ventilated because of infant respiratory distress syndrome. We administered two doses of 100 mg/kg each of a natural porcine surfactant with 13C-labeled dipalmitoylphosphatidylcholine as a tracer. The 13C enrichment of surfactant disaturated phosphatidylcholine (DSPC) was measured in serial tracheal aspirates by gas chromatography-mass spectrometry. The DSPC half-life was 34.2 ± 9.4 h (mean ± SD; range: 21.8 to 45.9 h). The apparent DSPC pool sizes were 5.8 ± 6.1 mg/kg (range: 0.1 to 17.0 mg/kg) and 17.3 ± 13.6 mg/kg (range: 3.3 to 41.0 mg/kg) at the time of the first and second surfactant doses, respectively. We present a novel and safe method that allows the tracing of exogenous surfactant phosphatidylcholine, the major lipid component of pulmonary surfactant, in infants who receive exogenous surfactant. This method could be a valuable tool for studying: (1) therapies that enhance lung/surfactant maturation; (2) the dosing and timing of surfactant therapy in different lung diseases; and (3) the comparison of different surfactant preparations.",
author = "Mirka Torresin and Zimmermann, {L. J I} and Cogo, {Paola E.} and Paola Cavicchioli and Tamara Badon and Giuseppe Giordano and Franco Zacchello and Sauer, {P. J J} and Carnielli, {Virgilio P.}",
year = "2000",
language = "English",
volume = "161",
pages = "1584--1589",
journal = "American Journal of Respiratory and Critical Care Medicine",
issn = "1073-449X",
publisher = "American Thoracic Society - AJRCCM",
number = "5",

}

TY - JOUR

T1 - Exogenous surfactant kinetics in infant respiratory distress syndrome

T2 - A novel method with stable isotopes

AU - Torresin, Mirka

AU - Zimmermann, L. J I

AU - Cogo, Paola E.

AU - Cavicchioli, Paola

AU - Badon, Tamara

AU - Giordano, Giuseppe

AU - Zacchello, Franco

AU - Sauer, P. J J

AU - Carnielli, Virgilio P.

PY - 2000

Y1 - 2000

N2 - Little is known about surfactant metabolism in newborn infants, since radioactive isotopes cannot be used in humans. We describe here a new method for studying exogenous surfactant pharmacokinetics in vivo. We measured surfactant half-life, pool size, and turnover time in eight preterm infants (gestational age: 30 ± 2 wk; birth weight: 1,416 ± 202 g) who were mechanically ventilated because of infant respiratory distress syndrome. We administered two doses of 100 mg/kg each of a natural porcine surfactant with 13C-labeled dipalmitoylphosphatidylcholine as a tracer. The 13C enrichment of surfactant disaturated phosphatidylcholine (DSPC) was measured in serial tracheal aspirates by gas chromatography-mass spectrometry. The DSPC half-life was 34.2 ± 9.4 h (mean ± SD; range: 21.8 to 45.9 h). The apparent DSPC pool sizes were 5.8 ± 6.1 mg/kg (range: 0.1 to 17.0 mg/kg) and 17.3 ± 13.6 mg/kg (range: 3.3 to 41.0 mg/kg) at the time of the first and second surfactant doses, respectively. We present a novel and safe method that allows the tracing of exogenous surfactant phosphatidylcholine, the major lipid component of pulmonary surfactant, in infants who receive exogenous surfactant. This method could be a valuable tool for studying: (1) therapies that enhance lung/surfactant maturation; (2) the dosing and timing of surfactant therapy in different lung diseases; and (3) the comparison of different surfactant preparations.

AB - Little is known about surfactant metabolism in newborn infants, since radioactive isotopes cannot be used in humans. We describe here a new method for studying exogenous surfactant pharmacokinetics in vivo. We measured surfactant half-life, pool size, and turnover time in eight preterm infants (gestational age: 30 ± 2 wk; birth weight: 1,416 ± 202 g) who were mechanically ventilated because of infant respiratory distress syndrome. We administered two doses of 100 mg/kg each of a natural porcine surfactant with 13C-labeled dipalmitoylphosphatidylcholine as a tracer. The 13C enrichment of surfactant disaturated phosphatidylcholine (DSPC) was measured in serial tracheal aspirates by gas chromatography-mass spectrometry. The DSPC half-life was 34.2 ± 9.4 h (mean ± SD; range: 21.8 to 45.9 h). The apparent DSPC pool sizes were 5.8 ± 6.1 mg/kg (range: 0.1 to 17.0 mg/kg) and 17.3 ± 13.6 mg/kg (range: 3.3 to 41.0 mg/kg) at the time of the first and second surfactant doses, respectively. We present a novel and safe method that allows the tracing of exogenous surfactant phosphatidylcholine, the major lipid component of pulmonary surfactant, in infants who receive exogenous surfactant. This method could be a valuable tool for studying: (1) therapies that enhance lung/surfactant maturation; (2) the dosing and timing of surfactant therapy in different lung diseases; and (3) the comparison of different surfactant preparations.

UR - http://www.scopus.com/inward/record.url?scp=0034109725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034109725&partnerID=8YFLogxK

M3 - Article

VL - 161

SP - 1584

EP - 1589

JO - American Journal of Respiratory and Critical Care Medicine

JF - American Journal of Respiratory and Critical Care Medicine

SN - 1073-449X

IS - 5

ER -