Exosomes, ectosomes and the two together. Physiology and pathology

Jacopo Meldolesi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Eukaryotic cells release to the extracellular space two types of vesicles which have attracted great interest, early on for their complex generation and function, and more recently for their role in multiple diseases including cancer. Previous studies have revealed ample information about these vesicles, especially the exosomes. In this review, I report the developments that have occurred during the last few years. Small vesicles first accumulated within large endocytic cisternae and are then converted into multivesicular bodies. Upon exocytosis of the latter, the released vesicles are defined as exosomes. Ectosomes, which are known only in scant detail, are larger vesicles that pinch off rapidly from the plasma membrane. The rate of this release increases markedly upon appropriate cell stimulation. Both types of vesicle are delimited by a membrane that delimits a condensed cargo composed by proteins, mRNAs, miRNAs, and DNA, which are sorted from the cytoplasm. Several proteins, previously presented as markers of either vesicle, are common to both. Today, only a few markers are accepted. Upon their release, both exosomes and ectosomes are addressed to specific target cells, where they bind and surface roll for several seconds. Later, vesicles undergo outside/in fusion to the plasma membrane by a process analogous to the fusions of retroviruses. Fusion results in the horizontal transfer of cargoes that govern changes of the genome and of protein expression, up to the reprogramming of cell structure and function. In many physiological functions, the two types of vesicles operate together. Their mixtures play specific roles in processes such as blood coagulation, angiogenesis, innate and acquired immunity, synaptic transmission. Currently, some pathology investigations are focused on the diagnosis of diseases through recognition of specific vesicles and on preliminary therapy with targeted vesicles loaded with molecules aggressive to diseased cells.

Original languageEnglish
Pages (from-to)171-180
Number of pages10
JournalForum on Immunopathological Diseases and Therapeutics
Volume6
Issue number3-4
DOIs
Publication statusPublished - 2015

Fingerprint

Cell-Derived Microparticles
Exosomes
Physiology
Pathology
Fusion reactions
Cell membranes
Cell Membrane
Multivesicular Bodies
Proteins
Exocytosis
Extracellular Space
Blood Coagulation
Eukaryotic Cells
Adaptive Immunity
Retroviridae
Coagulation
MicroRNAs
Innate Immunity
Synaptic Transmission
Cytoplasm

Keywords

  • Heterogeneity of extracellular vesicles
  • Horizontal transfer of cargoes
  • Luminal cargoes
  • Markers
  • Multivesicular bodies
  • Outside/inside membrane fusion
  • Plasma membrane pinch-off
  • Vesicle budding/rolling
  • Vesicle diagnosis
  • Vesicle therapy

ASJC Scopus subject areas

  • Genetics
  • Molecular Medicine
  • Biochemistry
  • Biotechnology

Cite this

Exosomes, ectosomes and the two together. Physiology and pathology. / Meldolesi, Jacopo.

In: Forum on Immunopathological Diseases and Therapeutics, Vol. 6, No. 3-4, 2015, p. 171-180.

Research output: Contribution to journalArticle

@article{ce846b41d85541d38c968da6fa22e1f2,
title = "Exosomes, ectosomes and the two together. Physiology and pathology",
abstract = "Eukaryotic cells release to the extracellular space two types of vesicles which have attracted great interest, early on for their complex generation and function, and more recently for their role in multiple diseases including cancer. Previous studies have revealed ample information about these vesicles, especially the exosomes. In this review, I report the developments that have occurred during the last few years. Small vesicles first accumulated within large endocytic cisternae and are then converted into multivesicular bodies. Upon exocytosis of the latter, the released vesicles are defined as exosomes. Ectosomes, which are known only in scant detail, are larger vesicles that pinch off rapidly from the plasma membrane. The rate of this release increases markedly upon appropriate cell stimulation. Both types of vesicle are delimited by a membrane that delimits a condensed cargo composed by proteins, mRNAs, miRNAs, and DNA, which are sorted from the cytoplasm. Several proteins, previously presented as markers of either vesicle, are common to both. Today, only a few markers are accepted. Upon their release, both exosomes and ectosomes are addressed to specific target cells, where they bind and surface roll for several seconds. Later, vesicles undergo outside/in fusion to the plasma membrane by a process analogous to the fusions of retroviruses. Fusion results in the horizontal transfer of cargoes that govern changes of the genome and of protein expression, up to the reprogramming of cell structure and function. In many physiological functions, the two types of vesicles operate together. Their mixtures play specific roles in processes such as blood coagulation, angiogenesis, innate and acquired immunity, synaptic transmission. Currently, some pathology investigations are focused on the diagnosis of diseases through recognition of specific vesicles and on preliminary therapy with targeted vesicles loaded with molecules aggressive to diseased cells.",
keywords = "Heterogeneity of extracellular vesicles, Horizontal transfer of cargoes, Luminal cargoes, Markers, Multivesicular bodies, Outside/inside membrane fusion, Plasma membrane pinch-off, Vesicle budding/rolling, Vesicle diagnosis, Vesicle therapy",
author = "Jacopo Meldolesi",
year = "2015",
doi = "10.1615/ForumImmunDisTher.2016015877",
language = "English",
volume = "6",
pages = "171--180",
journal = "Forum on Immunopathological Diseases and Therapeutics",
issn = "2151-8017",
publisher = "Begell House Inc.",
number = "3-4",

}

TY - JOUR

T1 - Exosomes, ectosomes and the two together. Physiology and pathology

AU - Meldolesi, Jacopo

PY - 2015

Y1 - 2015

N2 - Eukaryotic cells release to the extracellular space two types of vesicles which have attracted great interest, early on for their complex generation and function, and more recently for their role in multiple diseases including cancer. Previous studies have revealed ample information about these vesicles, especially the exosomes. In this review, I report the developments that have occurred during the last few years. Small vesicles first accumulated within large endocytic cisternae and are then converted into multivesicular bodies. Upon exocytosis of the latter, the released vesicles are defined as exosomes. Ectosomes, which are known only in scant detail, are larger vesicles that pinch off rapidly from the plasma membrane. The rate of this release increases markedly upon appropriate cell stimulation. Both types of vesicle are delimited by a membrane that delimits a condensed cargo composed by proteins, mRNAs, miRNAs, and DNA, which are sorted from the cytoplasm. Several proteins, previously presented as markers of either vesicle, are common to both. Today, only a few markers are accepted. Upon their release, both exosomes and ectosomes are addressed to specific target cells, where they bind and surface roll for several seconds. Later, vesicles undergo outside/in fusion to the plasma membrane by a process analogous to the fusions of retroviruses. Fusion results in the horizontal transfer of cargoes that govern changes of the genome and of protein expression, up to the reprogramming of cell structure and function. In many physiological functions, the two types of vesicles operate together. Their mixtures play specific roles in processes such as blood coagulation, angiogenesis, innate and acquired immunity, synaptic transmission. Currently, some pathology investigations are focused on the diagnosis of diseases through recognition of specific vesicles and on preliminary therapy with targeted vesicles loaded with molecules aggressive to diseased cells.

AB - Eukaryotic cells release to the extracellular space two types of vesicles which have attracted great interest, early on for their complex generation and function, and more recently for their role in multiple diseases including cancer. Previous studies have revealed ample information about these vesicles, especially the exosomes. In this review, I report the developments that have occurred during the last few years. Small vesicles first accumulated within large endocytic cisternae and are then converted into multivesicular bodies. Upon exocytosis of the latter, the released vesicles are defined as exosomes. Ectosomes, which are known only in scant detail, are larger vesicles that pinch off rapidly from the plasma membrane. The rate of this release increases markedly upon appropriate cell stimulation. Both types of vesicle are delimited by a membrane that delimits a condensed cargo composed by proteins, mRNAs, miRNAs, and DNA, which are sorted from the cytoplasm. Several proteins, previously presented as markers of either vesicle, are common to both. Today, only a few markers are accepted. Upon their release, both exosomes and ectosomes are addressed to specific target cells, where they bind and surface roll for several seconds. Later, vesicles undergo outside/in fusion to the plasma membrane by a process analogous to the fusions of retroviruses. Fusion results in the horizontal transfer of cargoes that govern changes of the genome and of protein expression, up to the reprogramming of cell structure and function. In many physiological functions, the two types of vesicles operate together. Their mixtures play specific roles in processes such as blood coagulation, angiogenesis, innate and acquired immunity, synaptic transmission. Currently, some pathology investigations are focused on the diagnosis of diseases through recognition of specific vesicles and on preliminary therapy with targeted vesicles loaded with molecules aggressive to diseased cells.

KW - Heterogeneity of extracellular vesicles

KW - Horizontal transfer of cargoes

KW - Luminal cargoes

KW - Markers

KW - Multivesicular bodies

KW - Outside/inside membrane fusion

KW - Plasma membrane pinch-off

KW - Vesicle budding/rolling

KW - Vesicle diagnosis

KW - Vesicle therapy

UR - http://www.scopus.com/inward/record.url?scp=84986226569&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84986226569&partnerID=8YFLogxK

U2 - 10.1615/ForumImmunDisTher.2016015877

DO - 10.1615/ForumImmunDisTher.2016015877

M3 - Article

VL - 6

SP - 171

EP - 180

JO - Forum on Immunopathological Diseases and Therapeutics

JF - Forum on Immunopathological Diseases and Therapeutics

SN - 2151-8017

IS - 3-4

ER -