TY - JOUR
T1 - Exosomes From Astrocyte Processes
T2 - Signaling to Neurons
AU - Venturini, Arianna
AU - Passalacqua, Mario
AU - Pelassa, Simone
AU - Pastorino, Fabio
AU - Tedesco, Mariateresa
AU - Cortese, Katia
AU - Gagliani, Maria Cristina
AU - Leo, Giuseppina
AU - Maura, Guido
AU - Guidolin, Diego
AU - Agnati, Luigi F
AU - Marcoli, Manuela
AU - Cervetto, Chiara
N1 - Copyright © 2019 Venturini, Passalacqua, Pelassa, Pastorino, Tedesco, Cortese, Gagliani, Leo, Maura, Guidolin, Agnati, Marcoli and Cervetto.
PY - 2019
Y1 - 2019
N2 - It is widely recognized that extracellular vesicles subserve non-classical signal transmission in the central nervous system. Here we assess if the astrocyte processes, that are recognized to play crucial roles in intercellular communication at the synapses and in neuron-astrocyte networks, could convey messages through extracellular vesicles. Our findings indicate, for the first time that freshly isolated astrocyte processes prepared from adult rat cerebral cortex, can indeed participate to signal transmission in central nervous system by releasing exosomes that by volume transmission might target near or long-distance sites. It is noteworthy that the exosomes released from the astrocyte processes proved ability to selectively target neurons. The astrocyte-derived exosomes were proven positive for neuroglobin, a protein functioning as neuroprotectant against cell insult; the possibility that exosomes might transfer neuroglobin to neurons would add a mechanism to the potential astrocytic neuroprotectant activity. Notably, the exosomes released from the processes of astrocytes maintained markers, which prove their parental astrocytic origin. This potentially allows the assessment of the cellular origin of exosomes that might be recovered from body fluids.
AB - It is widely recognized that extracellular vesicles subserve non-classical signal transmission in the central nervous system. Here we assess if the astrocyte processes, that are recognized to play crucial roles in intercellular communication at the synapses and in neuron-astrocyte networks, could convey messages through extracellular vesicles. Our findings indicate, for the first time that freshly isolated astrocyte processes prepared from adult rat cerebral cortex, can indeed participate to signal transmission in central nervous system by releasing exosomes that by volume transmission might target near or long-distance sites. It is noteworthy that the exosomes released from the astrocyte processes proved ability to selectively target neurons. The astrocyte-derived exosomes were proven positive for neuroglobin, a protein functioning as neuroprotectant against cell insult; the possibility that exosomes might transfer neuroglobin to neurons would add a mechanism to the potential astrocytic neuroprotectant activity. Notably, the exosomes released from the processes of astrocytes maintained markers, which prove their parental astrocytic origin. This potentially allows the assessment of the cellular origin of exosomes that might be recovered from body fluids.
U2 - 10.3389/fphar.2019.01452
DO - 10.3389/fphar.2019.01452
M3 - Article
C2 - 31849688
VL - 10
SP - 1452
JO - Frontiers in Pharmacology
JF - Frontiers in Pharmacology
SN - 1663-9812
ER -