TY - JOUR
T1 - Exosomes from human mesenchymal stem cells conduct aerobic metabolism in term and preterm newborn infants
AU - Panfoli, Isabella
AU - Ravera, Silvia
AU - Podestà, Marina
AU - Cossu, Claudia
AU - Santucci, Laura
AU - Bartolucci, Martina
AU - Bruschi, Maurizio
AU - Calzia, Daniela
AU - Sabatini, Federica
AU - Bruschettini, Matteo
AU - Ramenghi, Luca Antonio
AU - Romantsik, Olga
AU - Marimpietri, Danilo
AU - Pistoia, Vito
AU - Ghiggeri, Gianmarco
AU - Frassoni, Francesco
AU - Candiano, Giovanni
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Exosomes are secreted nanovesicles that are able to transfer RNA and proteins to target cells. The emerging role ofmesenchymal stemcell (MSC) exosomes as promoters of aerobic ATP synthesis restoration in damaged cells, prompted us to assess whether they contain an extramitochondrial aerobic respiration capacity. Exosomes were isolated from culture medium of human MSCs from umbilical cord of †37-wk-old newborns or between 28-to 30-wk-old newborns (i.e., term or preterm infants). Characterization of samples was conducted by cytofluorometry. Oxidative phosphorylation capacity was assessed by Western blot analysis, oximetry, and luminometric and fluorometric analyses.MSC exosomes express functional respiratory complexes I, IV, and V, consuming oxygen. ATP synthesis was only detectable in exosomes from term newborns, suggestive of a specific mechanism that is not completed at an early gestational age. Activities are outward facing and comparable to those detected in mitochondria isolated from term MSCs. MSC exosomes display an unsuspected aerobic respiratory ability independent of whole mitochondria. This may be relevant for their ability to rescue cell bioenergetics. The differential oxidativemetabolismof pretermvs. term exosomes sheds new light on the preterm newborn's clinical vulnerability. A reduced ability to repair damaged tissue and an increased capability to cope with anoxic environment for preterm infants can be envisaged.
AB - Exosomes are secreted nanovesicles that are able to transfer RNA and proteins to target cells. The emerging role ofmesenchymal stemcell (MSC) exosomes as promoters of aerobic ATP synthesis restoration in damaged cells, prompted us to assess whether they contain an extramitochondrial aerobic respiration capacity. Exosomes were isolated from culture medium of human MSCs from umbilical cord of †37-wk-old newborns or between 28-to 30-wk-old newborns (i.e., term or preterm infants). Characterization of samples was conducted by cytofluorometry. Oxidative phosphorylation capacity was assessed by Western blot analysis, oximetry, and luminometric and fluorometric analyses.MSC exosomes express functional respiratory complexes I, IV, and V, consuming oxygen. ATP synthesis was only detectable in exosomes from term newborns, suggestive of a specific mechanism that is not completed at an early gestational age. Activities are outward facing and comparable to those detected in mitochondria isolated from term MSCs. MSC exosomes display an unsuspected aerobic respiratory ability independent of whole mitochondria. This may be relevant for their ability to rescue cell bioenergetics. The differential oxidativemetabolismof pretermvs. term exosomes sheds new light on the preterm newborn's clinical vulnerability. A reduced ability to repair damaged tissue and an increased capability to cope with anoxic environment for preterm infants can be envisaged.
KW - ATP synthesis
KW - Electron transport chain
KW - Energy metabolism
KW - Oxidative phosphorylation
KW - Umbilical cord
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UR - http://www.scopus.com/inward/citedby.url?scp=84963894602&partnerID=8YFLogxK
U2 - 10.1096/fj.15-279679
DO - 10.1096/fj.15-279679
M3 - Article
C2 - 26655706
AN - SCOPUS:84963894602
VL - 30
SP - 1416
EP - 1424
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 4
ER -