Expanding human T regulatory cells with the mTOR-inhibitor rapamycin

Manuela Battaglia, Angela Stabilini, Eleonora Tresoldi

Research output: Chapter in Book/Report/Conference proceedingChapter


CD4 +CD25 +FOXP3 + T regulatory (Treg) cells are pivotal for the induction and maintenance of peripheral tolerance in both mice and humans. The possibility to use Treg cells for the treatment of T-cell-mediated diseases has recently gained increasing momentum. However, given the limited amount of circulating FOXP3 + Treg cells, efficient methods for their ex vivo expansion are highly desirable. Rapamycin allows for in vitro expansion of murine and human FOXP3 + Treg cells, which maintain their regulatory phenotype and suppressive capacity. Here, we describe in detail the powerful methods for enriching human FOXP3 + Treg cells starting from unfractionated CD4 + T cells or for expanding CD25 +-enriched Treg cells in the presence of rapamycin.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages15
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Immunological tolerance
  • Rapamycin
  • Regulatory T cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Battaglia, M., Stabilini, A., & Tresoldi, E. (2012). Expanding human T regulatory cells with the mTOR-inhibitor rapamycin. In Methods in Molecular Biology (Vol. 821, pp. 279-293). (Methods in Molecular Biology; Vol. 821). https://doi.org/10.1007/978-1-61779-430-8_17