Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting

Fulvio Magni, Cecilia Sarto, Cristina Valsecchi, Stefano Casellato, Stefano Ferrero Bogetto, Silvano Bosari, Andrea Di Fonzo, Roberto A. Perego, Matteo Corizzato, Giancarlo Doro, Carmen Galbusera, Francesco Rocco, Paolo Mocarelli, Marzia Galli Kienle

Research output: Contribution to journalArticle

Abstract

Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry, to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2-DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.

Original languageEnglish
Pages (from-to)816-825
Number of pages10
JournalProteomics
Volume5
Issue number3
DOIs
Publication statusPublished - Mar 2005

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Keywords

  • Human renal cortex
  • Kidney master image
  • Mass spectrometry
  • Two-dimensional gel electrophoresis; Peptide mass fingerprinting

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Magni, F., Sarto, C., Valsecchi, C., Casellato, S., Bogetto, S. F., Bosari, S., Di Fonzo, A., Perego, R. A., Corizzato, M., Doro, G., Galbusera, C., Rocco, F., Mocarelli, P., & Kienle, M. G. (2005). Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting. Proteomics, 5(3), 816-825. https://doi.org/10.1002/pmic.200401077