Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting

Fulvio Magni, Cecilia Sarto, Cristina Valsecchi, Stefano Casellato, Stefano Ferrero Bogetto, Silvano Bosari, Andrea Di Fonzo, Roberto A. Perego, Matteo Corizzato, Giancarlo Doro, Carmen Galbusera, Francesco Rocco, Paolo Mocarelli, Marzia Galli Kienle

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry, to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2-DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.

Original languageEnglish
Pages (from-to)816-825
Number of pages10
JournalProteomics
Volume5
Issue number3
DOIs
Publication statusPublished - Mar 2005

Fingerprint

Peptide Mapping
Electrophoresis, Gel, Two-Dimensional
Proteome
Electrophoresis
Gels
Kidney
Peptides
Proteomics
Proteins
Urologic Diseases
Nephrology
Mass spectrometry
Protein Isoforms
Display devices
Mass Spectrometry
Research

Keywords

  • Human renal cortex
  • Kidney master image
  • Mass spectrometry
  • Two-dimensional gel electrophoresis; Peptide mass fingerprinting

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting. / Magni, Fulvio; Sarto, Cecilia; Valsecchi, Cristina; Casellato, Stefano; Bogetto, Stefano Ferrero; Bosari, Silvano; Di Fonzo, Andrea; Perego, Roberto A.; Corizzato, Matteo; Doro, Giancarlo; Galbusera, Carmen; Rocco, Francesco; Mocarelli, Paolo; Kienle, Marzia Galli.

In: Proteomics, Vol. 5, No. 3, 03.2005, p. 816-825.

Research output: Contribution to journalArticle

Magni, F, Sarto, C, Valsecchi, C, Casellato, S, Bogetto, SF, Bosari, S, Di Fonzo, A, Perego, RA, Corizzato, M, Doro, G, Galbusera, C, Rocco, F, Mocarelli, P & Kienle, MG 2005, 'Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting', Proteomics, vol. 5, no. 3, pp. 816-825. https://doi.org/10.1002/pmic.200401077
Magni, Fulvio ; Sarto, Cecilia ; Valsecchi, Cristina ; Casellato, Stefano ; Bogetto, Stefano Ferrero ; Bosari, Silvano ; Di Fonzo, Andrea ; Perego, Roberto A. ; Corizzato, Matteo ; Doro, Giancarlo ; Galbusera, Carmen ; Rocco, Francesco ; Mocarelli, Paolo ; Kienle, Marzia Galli. / Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting. In: Proteomics. 2005 ; Vol. 5, No. 3. pp. 816-825.
@article{e5c107f24bbb472d8f17b481010659c0,
title = "Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting",
abstract = "Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry, to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2-DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.",
keywords = "Human renal cortex, Kidney master image, Mass spectrometry, Two-dimensional gel electrophoresis; Peptide mass fingerprinting",
author = "Fulvio Magni and Cecilia Sarto and Cristina Valsecchi and Stefano Casellato and Bogetto, {Stefano Ferrero} and Silvano Bosari and {Di Fonzo}, Andrea and Perego, {Roberto A.} and Matteo Corizzato and Giancarlo Doro and Carmen Galbusera and Francesco Rocco and Paolo Mocarelli and Kienle, {Marzia Galli}",
year = "2005",
month = "3",
doi = "10.1002/pmic.200401077",
language = "English",
volume = "5",
pages = "816--825",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "3",

}

TY - JOUR

T1 - Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting

AU - Magni, Fulvio

AU - Sarto, Cecilia

AU - Valsecchi, Cristina

AU - Casellato, Stefano

AU - Bogetto, Stefano Ferrero

AU - Bosari, Silvano

AU - Di Fonzo, Andrea

AU - Perego, Roberto A.

AU - Corizzato, Matteo

AU - Doro, Giancarlo

AU - Galbusera, Carmen

AU - Rocco, Francesco

AU - Mocarelli, Paolo

AU - Kienle, Marzia Galli

PY - 2005/3

Y1 - 2005/3

N2 - Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry, to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2-DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.

AB - Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry, to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2-DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.

KW - Human renal cortex

KW - Kidney master image

KW - Mass spectrometry

KW - Two-dimensional gel electrophoresis; Peptide mass fingerprinting

UR - http://www.scopus.com/inward/record.url?scp=20044388540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20044388540&partnerID=8YFLogxK

U2 - 10.1002/pmic.200401077

DO - 10.1002/pmic.200401077

M3 - Article

C2 - 15668995

AN - SCOPUS:20044388540

VL - 5

SP - 816

EP - 825

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 3

ER -