TY - JOUR
T1 - Expansion of major histocompatibility complex-restricted antimelanoma cytotoxic T-cell lymphocyte clones with identical T-cell receptor from tumor-infiltrating lymphocytes
AU - Sensi, Marialuisa
AU - Castelli, Chiara
AU - Salvi, Stefania
AU - Mazzocchi, Arabella
AU - Mortarini, Roberta
AU - Nicolini, Gabriella
AU - Anichini, Andrea
AU - Parmiani, Giorgio
PY - 1992
Y1 - 1992
N2 - Tumor-infiltrating lymphocytes (TILs) were isolated from a subcutaneous metastasis of melanoma and cytotoxic T-cell lymphocyte (CTL) lines were obtained by sensitizing in vitro four separate aliquots of TILs with autologous tumor cells and recombinant interleukin-2. All CTL lines were predominantly WT31+, CD3+, and CD8+ and displayed a preferential cytotoxic activity against the autologous tumor. T-cell receptor (TCR) composition was analyzed by using the polymerase chain reaction with 5' variable region (Vα or Vβ)-specific primers and 3' constant (Cα or Cβ) primers. The entire repertoire of the Vα and Vβ gene families tested was present in fresh TILs and in the CTL lines, although, in the latter, consistent quantitative variations in transcripts of several Vα and Vβ occurred. CTL clones that exhibited CD3-dependent and major histocompatibility complex-restricted killing of the autologous melanoma were isolated from the four TIL cultures. TCR analysis indicated that, independently from the culture of origin, only two combinations of Vα and Vβ gene families were present in the majority of these CTL clones. These Va and Vp gene families were not found in a panel of CTL clones that did not lyse the autologous tumor. This study indicates that recognition of melanoma antigens can strongly select for certain types of TCR-bearing T-lymphocytes.
AB - Tumor-infiltrating lymphocytes (TILs) were isolated from a subcutaneous metastasis of melanoma and cytotoxic T-cell lymphocyte (CTL) lines were obtained by sensitizing in vitro four separate aliquots of TILs with autologous tumor cells and recombinant interleukin-2. All CTL lines were predominantly WT31+, CD3+, and CD8+ and displayed a preferential cytotoxic activity against the autologous tumor. T-cell receptor (TCR) composition was analyzed by using the polymerase chain reaction with 5' variable region (Vα or Vβ)-specific primers and 3' constant (Cα or Cβ) primers. The entire repertoire of the Vα and Vβ gene families tested was present in fresh TILs and in the CTL lines, although, in the latter, consistent quantitative variations in transcripts of several Vα and Vβ occurred. CTL clones that exhibited CD3-dependent and major histocompatibility complex-restricted killing of the autologous melanoma were isolated from the four TIL cultures. TCR analysis indicated that, independently from the culture of origin, only two combinations of Vα and Vβ gene families were present in the majority of these CTL clones. These Va and Vp gene families were not found in a panel of CTL clones that did not lyse the autologous tumor. This study indicates that recognition of melanoma antigens can strongly select for certain types of TCR-bearing T-lymphocytes.
KW - Cytotoxic T-cell clones
KW - Melanoma
KW - T-cell receptor
KW - Tumor-infiltrating lymphocytes
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M3 - Article
C2 - 1332745
AN - SCOPUS:0026744871
VL - 12
SP - 207
EP - 211
JO - Journal of Immunotherapy
JF - Journal of Immunotherapy
SN - 1053-8550
IS - 3
ER -