Experiences in the measurement of RBC-bound IgG as markers of cell age

Renata Paleari, Ferruccio Ceriotti, Franco Azzario, Liliana Maccioni, Renzo Galanello, Andrea Mosca

Research output: Contribution to journalArticlepeer-review

Abstract

An immunologically mediated pathway has been largely accepted to be one of the mechanisms involved in the clearance of senescent or prematurely damaged RBC. According to this pathway, RBC removal is mediated by binding of naturally occurring IgG to clustered integral membrane proteins, followed by complement deposition. The validation of an immunoenzymatic method for the detection of RBC-bound autologous IgG is presented. The use of RBC-bound IgG as an index related to red cell age was evaluated by measuring IgG binding in RBC treated with the clustering agent ZnCl2, in density fractionated RBC and in a selected group of patients expected to have an altered RBC life span. The immunoenzymatic method for IgG detection resulted to be reproducible (CV=3.4%). IgG binding to in vitro clustered RBC was found to be enhanced to a very great extent, about 20 times higher with respect to untreated RBC. A slight but significant increase (about 1.8-fold) in membrane-bound IgG was observed in the highest density fraction of normal RBC, which constituted 1% of the total cells. A significantly greater number of RBC-bound IgG was measured in splenectomized β-thalassemia intermedia patients and in subjects with secondary decreases in the C3 complement fraction concentration.

Original languageEnglish
Pages (from-to)175-179
Number of pages5
JournalBioelectrochemistry
Volume62
Issue number2
DOIs
Publication statusPublished - May 2004

Keywords

  • β-Thalassemia intermedia
  • Aging
  • Complement
  • Erythrocytes
  • Naturally occurring antibodies

ASJC Scopus subject areas

  • Biophysics
  • Electrochemistry
  • Physical and Theoretical Chemistry

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