Experimental contamination assessment of a novel closed ultravitrification device

Enrique Criado, Federica Moalli, Nadia Polentarutti, Elena Albani, Giovanna Morreale, Francesca Menduni, Paolo Emanuele Levi-Setti

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To evaluate the safety of a new ultravitrification closed device. Design: Ultravitrification research. Setting: Private assisted reproduction center. Animal(s): Microorganisms (bacteria). Intervention(s): A styrofoam container holding 1,000 mL of liquid nitrogen (LN2) was contaminated with Pseudomonas aeruginosa and Escherichia coli. Forty closed devices (Ultravit) and 20 open devices (Cryotop) loading approximately 0.5 μL of antibiotic-free medium were plunged into this contaminated LN2 for 5-10 seconds and then inoculated into selective culture dishes. Colony-forming units were analyzed and counted after an overnight incubation at 37°C. Main Outcome Measure(s): Detection of micro-organisms in different devices after incubation. Result(s): There was no contamination in any of the closed devices, whereas in 45% of open devices these bacteria were present. Conclusion(s): With this study we demonstrated, in an experimental model using contaminated LN2, that this new closed device is a safe system that does not allow cell contact with LN2, avoiding cell contamination.

Original languageEnglish
Pages (from-to)1777-1779
Number of pages3
JournalFertility and Sterility
Volume95
Issue number5
DOIs
Publication statusPublished - Apr 2011

Keywords

  • bacteria
  • Contamination
  • cross-contamination
  • cryopreservation
  • oocyte ultravitrification
  • risk
  • Ultravit

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

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