Abstract
Objective: To evaluate the safety of a new ultravitrification closed device. Design: Ultravitrification research. Setting: Private assisted reproduction center. Animal(s): Microorganisms (bacteria). Intervention(s): A styrofoam container holding 1,000 mL of liquid nitrogen (LN2) was contaminated with Pseudomonas aeruginosa and Escherichia coli. Forty closed devices (Ultravit) and 20 open devices (Cryotop) loading approximately 0.5 μL of antibiotic-free medium were plunged into this contaminated LN2 for 5-10 seconds and then inoculated into selective culture dishes. Colony-forming units were analyzed and counted after an overnight incubation at 37°C. Main Outcome Measure(s): Detection of micro-organisms in different devices after incubation. Result(s): There was no contamination in any of the closed devices, whereas in 45% of open devices these bacteria were present. Conclusion(s): With this study we demonstrated, in an experimental model using contaminated LN2, that this new closed device is a safe system that does not allow cell contact with LN2, avoiding cell contamination.
| Original language | English |
|---|---|
| Pages (from-to) | 1777-1779 |
| Number of pages | 3 |
| Journal | Fertility and Sterility |
| Volume | 95 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Apr 2011 |
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Keywords
- bacteria
- Contamination
- cross-contamination
- cryopreservation
- oocyte ultravitrification
- risk
- Ultravit
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Reproductive Medicine
Cite this
Experimental contamination assessment of a novel closed ultravitrification device. / Criado, Enrique; Moalli, Federica; Polentarutti, Nadia; Albani, Elena; Morreale, Giovanna; Menduni, Francesca; Levi-Setti, Paolo Emanuele.
In: Fertility and Sterility, Vol. 95, No. 5, 04.2011, p. 1777-1779.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Experimental contamination assessment of a novel closed ultravitrification device
AU - Criado, Enrique
AU - Moalli, Federica
AU - Polentarutti, Nadia
AU - Albani, Elena
AU - Morreale, Giovanna
AU - Menduni, Francesca
AU - Levi-Setti, Paolo Emanuele
PY - 2011/4
Y1 - 2011/4
N2 - Objective: To evaluate the safety of a new ultravitrification closed device. Design: Ultravitrification research. Setting: Private assisted reproduction center. Animal(s): Microorganisms (bacteria). Intervention(s): A styrofoam container holding 1,000 mL of liquid nitrogen (LN2) was contaminated with Pseudomonas aeruginosa and Escherichia coli. Forty closed devices (Ultravit) and 20 open devices (Cryotop) loading approximately 0.5 μL of antibiotic-free medium were plunged into this contaminated LN2 for 5-10 seconds and then inoculated into selective culture dishes. Colony-forming units were analyzed and counted after an overnight incubation at 37°C. Main Outcome Measure(s): Detection of micro-organisms in different devices after incubation. Result(s): There was no contamination in any of the closed devices, whereas in 45% of open devices these bacteria were present. Conclusion(s): With this study we demonstrated, in an experimental model using contaminated LN2, that this new closed device is a safe system that does not allow cell contact with LN2, avoiding cell contamination.
AB - Objective: To evaluate the safety of a new ultravitrification closed device. Design: Ultravitrification research. Setting: Private assisted reproduction center. Animal(s): Microorganisms (bacteria). Intervention(s): A styrofoam container holding 1,000 mL of liquid nitrogen (LN2) was contaminated with Pseudomonas aeruginosa and Escherichia coli. Forty closed devices (Ultravit) and 20 open devices (Cryotop) loading approximately 0.5 μL of antibiotic-free medium were plunged into this contaminated LN2 for 5-10 seconds and then inoculated into selective culture dishes. Colony-forming units were analyzed and counted after an overnight incubation at 37°C. Main Outcome Measure(s): Detection of micro-organisms in different devices after incubation. Result(s): There was no contamination in any of the closed devices, whereas in 45% of open devices these bacteria were present. Conclusion(s): With this study we demonstrated, in an experimental model using contaminated LN2, that this new closed device is a safe system that does not allow cell contact with LN2, avoiding cell contamination.
KW - bacteria
KW - Contamination
KW - cross-contamination
KW - cryopreservation
KW - oocyte ultravitrification
KW - risk
KW - Ultravit
UR - http://www.scopus.com/inward/record.url?scp=79952988317&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952988317&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2010.12.044
DO - 10.1016/j.fertnstert.2010.12.044
M3 - Article
C2 - 21269610
AN - SCOPUS:79952988317
VL - 95
SP - 1777
EP - 1779
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 5
ER -