Experimental Goodpasture's syndrome in Wistar-Kyoto rats immunized with α3 chain of type IV collagen

Mauro Abbate, Raghuram Kalluri, Daniela Corna, Naoto Yamaguchi, Robert T. Mccluskey, Billy G. Hudson, Giuseppe Andres, Carla Zoja, Giuseppe Remuzzi

Research output: Contribution to journalArticle

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Abstract

Background. Glomerulonephritis and tung hemorrhage of autoimmune Goodpasture syndrome develop due to immune reactions against epitope(s) of the non-collagenous (NC1) domain of α3-chain of type IV collagen [α3(IV) NC1]. Whether thymic mechanisms have a role in the loss of tolerance to the Goodpasture epitope has not been established. We studied the renal and pulmonary effects of immunization with different forms (monomer, dimer, or hexamer) of α3(IV) NC1 collagen in Wistar-Kyoto (WKY) rats, and assessed whether the intrathymic inoculation of the antigen may protect against anti- GBM disease. Methods. WKY rats were immunized with bovine α3(IV) monomer, dimer, or hexamer, or with α3(IV) NC1 synthetic peptide. Renal function, kidney and lung immunohistology, and circulating and tissue bound antibodies to type IV collagen chains were analyzed. Effects of intrathymic inoculation of antigen on subsequent disease induction were analyzed in WKY rats given α3(IV) NC1 dimer or GBM preparation intrathymically 48 hours before immunization. Results. Proteinuria, linear IgG deposition in GBM, and crescentic glomerulonephritis developed in WKY rats immunized with α3(IV) NC1 dimer or hexamer. Lesions were dose-dependent upon injections of 10 to 100 μg dimer. The α3(IV) NC1 monomer induced less severe proteinuria and no crescents. Pulmonary hemorrhage was detectable in 35% of rats immunized with 25 to 100 μg α3(IV) NC1 dimer; α3(IV) synthetic peptide (36 carboxyl terminal) did not induce disease. Rats injected intrathymically with up to 100 μg α3(IV) NC1 dimer or with GBM 48 hours before immunization were not protected against subsequent development of proteinuria and glomerulonephritis. Conclusions. These findings document that glomerulonephritis and lung hemorrhage can be elicited in WKY rats by immunization with α3(IV) NC1. Failure of the intrathymic inoculation of antigen to prevent disease suggests that immunological tolerance cannot be achieved by this intervention, in contrast to other autoimmune conditions, and may imply independent roles for cellular and humoral nephritogenic pathways in anti-GBM nephritis.

Original languageEnglish
Pages (from-to)1550-1561
Number of pages12
JournalKidney International
Volume54
Issue number5
DOIs
Publication statusPublished - 1998

Fingerprint

Anti-Glomerular Basement Membrane Disease
Collagen Type IV
Inbred WKY Rats
Glomerulonephritis
Immunization
Proteinuria
Lung
Hemorrhage
Kidney
Antigens
Epitopes
Aleurites
Peptides
Collagen Type III
Nephritis
Immune System Diseases
Collagen
Immunoglobulin G
Injections
Antibodies

Keywords

  • Anti-GBM disease
  • Dendritic cell
  • Extracellular matrix
  • GBM α3(IV) NC1 peptide
  • Glomerulonephritis
  • Lung hemorrhage
  • Thymocyte

ASJC Scopus subject areas

  • Nephrology

Cite this

Experimental Goodpasture's syndrome in Wistar-Kyoto rats immunized with α3 chain of type IV collagen. / Abbate, Mauro; Kalluri, Raghuram; Corna, Daniela; Yamaguchi, Naoto; Mccluskey, Robert T.; Hudson, Billy G.; Andres, Giuseppe; Zoja, Carla; Remuzzi, Giuseppe.

In: Kidney International, Vol. 54, No. 5, 1998, p. 1550-1561.

Research output: Contribution to journalArticle

Abbate, Mauro ; Kalluri, Raghuram ; Corna, Daniela ; Yamaguchi, Naoto ; Mccluskey, Robert T. ; Hudson, Billy G. ; Andres, Giuseppe ; Zoja, Carla ; Remuzzi, Giuseppe. / Experimental Goodpasture's syndrome in Wistar-Kyoto rats immunized with α3 chain of type IV collagen. In: Kidney International. 1998 ; Vol. 54, No. 5. pp. 1550-1561.
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abstract = "Background. Glomerulonephritis and tung hemorrhage of autoimmune Goodpasture syndrome develop due to immune reactions against epitope(s) of the non-collagenous (NC1) domain of α3-chain of type IV collagen [α3(IV) NC1]. Whether thymic mechanisms have a role in the loss of tolerance to the Goodpasture epitope has not been established. We studied the renal and pulmonary effects of immunization with different forms (monomer, dimer, or hexamer) of α3(IV) NC1 collagen in Wistar-Kyoto (WKY) rats, and assessed whether the intrathymic inoculation of the antigen may protect against anti- GBM disease. Methods. WKY rats were immunized with bovine α3(IV) monomer, dimer, or hexamer, or with α3(IV) NC1 synthetic peptide. Renal function, kidney and lung immunohistology, and circulating and tissue bound antibodies to type IV collagen chains were analyzed. Effects of intrathymic inoculation of antigen on subsequent disease induction were analyzed in WKY rats given α3(IV) NC1 dimer or GBM preparation intrathymically 48 hours before immunization. Results. Proteinuria, linear IgG deposition in GBM, and crescentic glomerulonephritis developed in WKY rats immunized with α3(IV) NC1 dimer or hexamer. Lesions were dose-dependent upon injections of 10 to 100 μg dimer. The α3(IV) NC1 monomer induced less severe proteinuria and no crescents. Pulmonary hemorrhage was detectable in 35{\%} of rats immunized with 25 to 100 μg α3(IV) NC1 dimer; α3(IV) synthetic peptide (36 carboxyl terminal) did not induce disease. Rats injected intrathymically with up to 100 μg α3(IV) NC1 dimer or with GBM 48 hours before immunization were not protected against subsequent development of proteinuria and glomerulonephritis. Conclusions. These findings document that glomerulonephritis and lung hemorrhage can be elicited in WKY rats by immunization with α3(IV) NC1. Failure of the intrathymic inoculation of antigen to prevent disease suggests that immunological tolerance cannot be achieved by this intervention, in contrast to other autoimmune conditions, and may imply independent roles for cellular and humoral nephritogenic pathways in anti-GBM nephritis.",
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AU - Abbate, Mauro

AU - Kalluri, Raghuram

AU - Corna, Daniela

AU - Yamaguchi, Naoto

AU - Mccluskey, Robert T.

AU - Hudson, Billy G.

AU - Andres, Giuseppe

AU - Zoja, Carla

AU - Remuzzi, Giuseppe

PY - 1998

Y1 - 1998

N2 - Background. Glomerulonephritis and tung hemorrhage of autoimmune Goodpasture syndrome develop due to immune reactions against epitope(s) of the non-collagenous (NC1) domain of α3-chain of type IV collagen [α3(IV) NC1]. Whether thymic mechanisms have a role in the loss of tolerance to the Goodpasture epitope has not been established. We studied the renal and pulmonary effects of immunization with different forms (monomer, dimer, or hexamer) of α3(IV) NC1 collagen in Wistar-Kyoto (WKY) rats, and assessed whether the intrathymic inoculation of the antigen may protect against anti- GBM disease. Methods. WKY rats were immunized with bovine α3(IV) monomer, dimer, or hexamer, or with α3(IV) NC1 synthetic peptide. Renal function, kidney and lung immunohistology, and circulating and tissue bound antibodies to type IV collagen chains were analyzed. Effects of intrathymic inoculation of antigen on subsequent disease induction were analyzed in WKY rats given α3(IV) NC1 dimer or GBM preparation intrathymically 48 hours before immunization. Results. Proteinuria, linear IgG deposition in GBM, and crescentic glomerulonephritis developed in WKY rats immunized with α3(IV) NC1 dimer or hexamer. Lesions were dose-dependent upon injections of 10 to 100 μg dimer. The α3(IV) NC1 monomer induced less severe proteinuria and no crescents. Pulmonary hemorrhage was detectable in 35% of rats immunized with 25 to 100 μg α3(IV) NC1 dimer; α3(IV) synthetic peptide (36 carboxyl terminal) did not induce disease. Rats injected intrathymically with up to 100 μg α3(IV) NC1 dimer or with GBM 48 hours before immunization were not protected against subsequent development of proteinuria and glomerulonephritis. Conclusions. These findings document that glomerulonephritis and lung hemorrhage can be elicited in WKY rats by immunization with α3(IV) NC1. Failure of the intrathymic inoculation of antigen to prevent disease suggests that immunological tolerance cannot be achieved by this intervention, in contrast to other autoimmune conditions, and may imply independent roles for cellular and humoral nephritogenic pathways in anti-GBM nephritis.

AB - Background. Glomerulonephritis and tung hemorrhage of autoimmune Goodpasture syndrome develop due to immune reactions against epitope(s) of the non-collagenous (NC1) domain of α3-chain of type IV collagen [α3(IV) NC1]. Whether thymic mechanisms have a role in the loss of tolerance to the Goodpasture epitope has not been established. We studied the renal and pulmonary effects of immunization with different forms (monomer, dimer, or hexamer) of α3(IV) NC1 collagen in Wistar-Kyoto (WKY) rats, and assessed whether the intrathymic inoculation of the antigen may protect against anti- GBM disease. Methods. WKY rats were immunized with bovine α3(IV) monomer, dimer, or hexamer, or with α3(IV) NC1 synthetic peptide. Renal function, kidney and lung immunohistology, and circulating and tissue bound antibodies to type IV collagen chains were analyzed. Effects of intrathymic inoculation of antigen on subsequent disease induction were analyzed in WKY rats given α3(IV) NC1 dimer or GBM preparation intrathymically 48 hours before immunization. Results. Proteinuria, linear IgG deposition in GBM, and crescentic glomerulonephritis developed in WKY rats immunized with α3(IV) NC1 dimer or hexamer. Lesions were dose-dependent upon injections of 10 to 100 μg dimer. The α3(IV) NC1 monomer induced less severe proteinuria and no crescents. Pulmonary hemorrhage was detectable in 35% of rats immunized with 25 to 100 μg α3(IV) NC1 dimer; α3(IV) synthetic peptide (36 carboxyl terminal) did not induce disease. Rats injected intrathymically with up to 100 μg α3(IV) NC1 dimer or with GBM 48 hours before immunization were not protected against subsequent development of proteinuria and glomerulonephritis. Conclusions. These findings document that glomerulonephritis and lung hemorrhage can be elicited in WKY rats by immunization with α3(IV) NC1. Failure of the intrathymic inoculation of antigen to prevent disease suggests that immunological tolerance cannot be achieved by this intervention, in contrast to other autoimmune conditions, and may imply independent roles for cellular and humoral nephritogenic pathways in anti-GBM nephritis.

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KW - Dendritic cell

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KW - GBM α3(IV) NC1 peptide

KW - Glomerulonephritis

KW - Lung hemorrhage

KW - Thymocyte

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