Protocollo sperimentale in vivo per la valutazione dell'effetto di miscele di O2/O3 a diverse concentrazioni sul disco intervertebrale

Translated title of the contribution: Experimental in vivo protocol to study the effect of O2/O3 mixtures at different concentrations on the intervertebral disc

M. Brayda-Bruno, M. Fini, M. Rocca, G. Giavaresi, R. Giardino

Research output: Contribution to journalArticlepeer-review

Abstract

Oxygen-ozone (O2/O3) therapy has an empirical clinical use as a non-surgical technique for the treatment of herniated disks by means of injections both in the disk and in the paravertebral muscles. Even if good clinical results are obtained by this technique, to our knowledge, few experimental studies exist on the rationale, mechanism of action and possible toxicity of O2/O3 administration, probably also because of some difficulties due to the in vivo O3 measurement. The aim of the present study is the development of a reliable animal model to better understand: 1) the rationale that could explain the clinical success; 2) the relation between administered doses and discal tissue damage related to the disappearance of symptoms; 3) toxicity dose in animals. After a preliminary evaluation of different animal species, also on the basis of previous experiences of our group, the sheep was selected as a useful model because of size and anatomic characteristics of the cervical spine. By strictly following European and Italian Laws on animal experimentation (under general anaesthesia and with pre and post-operative veterinarian animal care), the experimental protocol will be carried out by performing the injection in three cervical intervertebral disks of oxygen (O2), O2/O3 at a dosage of 30 μg/ml and O2/O3 at a dosage of 70 μg/ml. At different experimental times (2, 7 and 30 days after surgery), under general anaesthesia, animals will be killed and after an accurate macroscopic examination of discal and paravertebral tissues, histological, morphometric, histochemical and ultrastructural studies will be performed particularly on discal collagen fibres and proteoglycans. In some other animals the injections will be executed in the paravertebral muscular tissues for 30 days every 4-5 days and the possible macroscopic and microscopic modifications on local and discal tissues will be studied.

Translated title of the contributionExperimental in vivo protocol to study the effect of O2/O3 mixtures at different concentrations on the intervertebral disc
Original languageItalian
Pages (from-to)31-33
Number of pages3
JournalRivista di Neuroradiologia
Volume14
Issue numberSUPPL. 1
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Clinical Neurology
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

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