Abstract
Honey is widely used for treating wounds, burns and ulcers, due to its antimicrobial and anti-inflammatory properties. Recently, it has been shown that it can also promote tissue repair. However, the mechanisms of action in the wound healing process are still far from full comprehension. In this work we propose an experimental methodology to investigate the potential role of honey as wound repair enhancer, by analyzing in vitro cell motility and proliferation, that are the main mechanisms involved in the wound repair. Our methodological approach is based on the well-assessed in vitro Wound Healing (WH) assay, coupled with Time-Lapse Microscopy (TLM) and image analysis techniques. Our methodology was applied to test the potential healing activities of several kinds of honeys and mixtures of selected honeys, on monolayers of HT1080 human fibrosarcoma cells. The honeys were also characterized for their antimicrobial activity. Overall, this work is focused on raising new therapeutic interest in the valuable natural product honey.
| Original language | English |
|---|---|
| Title of host publication | Chemical Engineering Transactions |
| Publisher | Italian Association of Chemical Engineering - AIDIC |
| Pages | 259-264 |
| Number of pages | 6 |
| Volume | 49 |
| ISBN (Print) | 9788895608402 |
| DOIs | |
| Publication status | Published - Apr 1 2016 |
| Externally published | Yes |
Publication series
| Name | Chemical Engineering Transactions |
|---|---|
| Volume | 49 |
| ISSN (Electronic) | 22839216 |
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ASJC Scopus subject areas
- Chemical Engineering(all)
Cite this
Experimental investigation of honey as wound repair enhancer by in vitro time-lapse microscopy. / Ascione, Flora; Zuorro, Antonio; Fidaleo, Marco; Lavecchia, Roberto; Guido, Stefano; Caserta, Sergio.
Chemical Engineering Transactions. Vol. 49 Italian Association of Chemical Engineering - AIDIC, 2016. p. 259-264 (Chemical Engineering Transactions; Vol. 49).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Experimental investigation of honey as wound repair enhancer by in vitro time-lapse microscopy
AU - Ascione, Flora
AU - Zuorro, Antonio
AU - Fidaleo, Marco
AU - Lavecchia, Roberto
AU - Guido, Stefano
AU - Caserta, Sergio
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Honey is widely used for treating wounds, burns and ulcers, due to its antimicrobial and anti-inflammatory properties. Recently, it has been shown that it can also promote tissue repair. However, the mechanisms of action in the wound healing process are still far from full comprehension. In this work we propose an experimental methodology to investigate the potential role of honey as wound repair enhancer, by analyzing in vitro cell motility and proliferation, that are the main mechanisms involved in the wound repair. Our methodological approach is based on the well-assessed in vitro Wound Healing (WH) assay, coupled with Time-Lapse Microscopy (TLM) and image analysis techniques. Our methodology was applied to test the potential healing activities of several kinds of honeys and mixtures of selected honeys, on monolayers of HT1080 human fibrosarcoma cells. The honeys were also characterized for their antimicrobial activity. Overall, this work is focused on raising new therapeutic interest in the valuable natural product honey.
AB - Honey is widely used for treating wounds, burns and ulcers, due to its antimicrobial and anti-inflammatory properties. Recently, it has been shown that it can also promote tissue repair. However, the mechanisms of action in the wound healing process are still far from full comprehension. In this work we propose an experimental methodology to investigate the potential role of honey as wound repair enhancer, by analyzing in vitro cell motility and proliferation, that are the main mechanisms involved in the wound repair. Our methodological approach is based on the well-assessed in vitro Wound Healing (WH) assay, coupled with Time-Lapse Microscopy (TLM) and image analysis techniques. Our methodology was applied to test the potential healing activities of several kinds of honeys and mixtures of selected honeys, on monolayers of HT1080 human fibrosarcoma cells. The honeys were also characterized for their antimicrobial activity. Overall, this work is focused on raising new therapeutic interest in the valuable natural product honey.
UR - http://www.scopus.com/inward/record.url?scp=84969544821&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84969544821&partnerID=8YFLogxK
U2 - 10.3303/CET1649044
DO - 10.3303/CET1649044
M3 - Chapter
SN - 9788895608402
VL - 49
T3 - Chemical Engineering Transactions
SP - 259
EP - 264
BT - Chemical Engineering Transactions
PB - Italian Association of Chemical Engineering - AIDIC
ER -