Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics

Alice Di Pierro, Heather Bondi, Chiara Monti, Luisa Pieroni, Enrico Cilio, Andrea Urbani, Tiziana Alberio, Mauro Fasano, Maurizio Ronci

Research output: Contribution to journalArticle

Abstract

Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences.

Original languageEnglish
Pages (from-to)1-3
Number of pages3
JournalEuPA Open Proteomics
Volume11
DOIs
Publication statusPublished - Jun 1 2016

Keywords

  • Electroelution
  • Mitochondria
  • Protease
  • Shotgun
  • Top-down

ASJC Scopus subject areas

  • Biochemistry

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