Experimental study on the efficacy of combination of α-helical antimicrobial peptides and vancomycin against Staphylococcus aureus with intermediate resistance to glycopeptides

Oscar Cirioni, Carmela Silvestri, Roberto Ghiselli, Andrea Giacometti, Fiorenza Orlando, Federico Mocchegiani, Leonardo Chiodi, Agnese Della Vittoria, Vittorio Saba, Giorgio Scalise

Research output: Contribution to journalArticle

Abstract

An experimental study has been performed to compare the in vitro activity and the in vivo efficacy of magainin II and cecropin A, two α-helical antimicrobial peptides, and vancomycin against Staphylococcus aureus with intermediate resistance to glycopeptides. In vitro experiments included MIC determination, time-kill and synergy studies. For in vivo studies, a mouse model of staphylococcal sepsis has been used. Main outcome measures were: lethality, quantitative blood cultures and detection of TNF-alpha and interleukin-6 (IL-6) plasma levels. Combinations of α-helical antimicrobial peptides showed in vitro synergistic interaction. Significant increase in efficacy was also observed in vivo: combined-treated groups had significant lower bacteremia when compared to single-treated groups. Magainin II combined with vancomycin exhibited the highest efficacy on all main outcome measurements. These results highlight the potential usefulness of these combinations and provide future therapeutic alternative in infections due to glycopeptides resistant staphylococci in the coming years.

Original languageEnglish
Pages (from-to)2600-2606
Number of pages7
JournalPeptides
Volume27
Issue number11
DOIs
Publication statusPublished - Nov 2006

Keywords

  • Antimicrobial peptides
  • Glycopeptides
  • Staphylococcal sepsis
  • Staphylococci

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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