Experimental subarachnoid hemorrhage - Lipid peroxidation and Na+, K+-ATPase in different rat brain areas

Fulvio Marzatico, Paolo Gaetani, Riccardo Rodriguez y Baena, Vittorio Silvani, Ilaria Fulle, Daniela Lombardi, Paolo Ferlenga, Gianni Benzi

Research output: Contribution to journalArticle

Abstract

Subarachnoid hemorrhage (SAH) was produced in Sprague Dawley rats by injection of 0.30 mL of autologous arterial blood into the cisterna magna. Tissue lipid peroxide, quantified as thiobarbituric acid reactive material (TBAR), and Na+, K+-ATPase activity were assayed in three different rat brain areas (cerebral cortex, hippocampus, and brain stem) of sham-operated rats and in four hemorrhagic rat groups at 30 min, 1 h, 6h, and 2 d after SAH. Na+, K+-ATPase activity decreased in the cerebral cortex at 30 min, 1 h, and 6 h and in the brain stem at 1 h after SAH induction, whereas enzymatic activity was unchanged in the hippocampus. There was no evident difference in lipid peroxide content between sham-operated animals and hemorrhagic animals. These results indicate that little modifications in lipid peroxidative process (as expressed in TBAR) are not responsible for changes in the ATPase activity.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalMolecular and Chemical Neuropathology
Volume11
Issue number2
DOIs
Publication statusPublished - Oct 1989

Keywords

  • lipid peroxidation
  • malonyldialdehyde
  • Na, K-ATPase
  • Subarachnoid hemorrhage
  • vasospasm

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Molecular Biology

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