Exploitation of a library of alpha-galactosidases for the synthesis of building blocks for glycopolymers.

Monica Casali, Letizia Tarantini, Sergio Riva, Zdenka Hunkova, Lenka Weignerova, Vladimir Kren

Research output: Contribution to journalArticlepeer-review

Abstract

After screening a library of fungal alpha-galactosidases for the synthesis of functionalized alkyl alpha-D-galactopyranosides, four enzymes (isolated from Aspergillus terreus CCM55, Aspergillus commune CCM 2969, Penicillium vinaceum CCM 2384, or Penicillium brasilianum 2155) proved to be suitable for these biotransformations. The effect of different concentrations of alcohol on activity and stability of these enzymes was investigated. After optimization of the reaction conditions, three galactose derivatives (allyl, 2-nitroethyl and 2-(2',2',2'-trifluoroacetamido)-ethyl alpha-D-galactopyranoside, 1a, 3a, and 4a, respectively), suitable for subsequent chemical polymerization, were synthesized using either the "reverse hydrolysis" or the "transglycosylation" protocols.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalBiotechnology and Bioengineering
Volume77
Issue number1
DOIs
Publication statusPublished - Jan 5 2002

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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