Exploiting microRNA regulation for genetic engineering

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

RNA interference (RNAi) has been a landmark discovery in science. A typical application is to knock down the expression of endogenous genes by delivering small interfering RNA (siRNA) into cells triggering the degradation of complementary mRNA. However, RNAi can also be exploited the other way round: making use of the huge diversity of endogenous microRNAs (miRNA), the expression of exogenously introduced genes tagged with artificial miRNA target sequences can be negatively regulated according to the activity of a given miRNA which can be tissue-, lineage-, activation- or differentiation stage specific. This has significantly expanded the regulatory potential of gene transfer vectors and will benefit both basic science and therapeutic applications. This review briefly introduces the reader to the technical basis for exploiting miRNA regulation, followed by a discussion of specific applications for miRNA-regulated vectors/viruses in basic research, gene- and virotherapy.

Original languageEnglish
Pages (from-to)393-403
Number of pages11
JournalTissue Antigens
Volume80
Issue number5
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Genetic engineering
Genetic Engineering
MicroRNAs
Genes
RNA Interference
RNA
Gene transfer
RNA Stability
Regulator Genes
Viruses
Small Interfering RNA
Chemical activation
Tissue
Gene Expression
Degradation
Messenger RNA
Research

Keywords

  • Gene therapy
  • microRNA regulated vectors
  • miRNA regulation
  • Post-transcriptional detargeting

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Genetics

Cite this

Exploiting microRNA regulation for genetic engineering. / Gentner, B.; Naldini, L.

In: Tissue Antigens, Vol. 80, No. 5, 11.2012, p. 393-403.

Research output: Contribution to journalArticle

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