Exploring myelin basic protein for HLA class I-binding sequences

Nobuyuki Tanigaki, Doriana Fruci, Niegel Groome, Richard H. Butler, Marco Londei, Roberto Tosi

Research output: Contribution to journalArticle

Abstract

In view of the increasing evidence of the involvement of CD8+ T cells in the pathogenesis of multiple sclerosis (MS), we have scanned the sequence of the myelin basic protein (MBP), using 162 overlapping nonapeptides, for HLA-class I binding sites. Peptide binding was measured using the recently reported HLA class I α-chain-refolding assay, and the following HLA allelic products were analyzed: HLA-A2 ((*)0201, (*)0204), B27 ((*)2705), B35, B51 and B62. A considerable number of binding peptides were distinguished for each of the allelic products tested. In addition, three interesting points emerged. The first was the identification of several binding peptides which did not contain the known anchor motifs. The second was the evidence that several peptides showed a promiscuous binding profile, being able to bind to different HLA class I molecules that were either allelic or non allelic. The third was that in several cases two consecutive peptides could bind to the same HLA molecule.

Original languageEnglish
Pages (from-to)2196-2202
Number of pages7
JournalEuropean Journal of Immunology
Volume24
Issue number9
Publication statusPublished - Sep 1994

Fingerprint

Myelin Basic Protein
Peptides
HLA-A2 Antigen
Multiple Sclerosis
Binding Sites
T-Lymphocytes

Keywords

  • HLA class I
  • HLA-peptide binding
  • Myelin basic protein

ASJC Scopus subject areas

  • Immunology

Cite this

Tanigaki, N., Fruci, D., Groome, N., Butler, R. H., Londei, M., & Tosi, R. (1994). Exploring myelin basic protein for HLA class I-binding sequences. European Journal of Immunology, 24(9), 2196-2202.

Exploring myelin basic protein for HLA class I-binding sequences. / Tanigaki, Nobuyuki; Fruci, Doriana; Groome, Niegel; Butler, Richard H.; Londei, Marco; Tosi, Roberto.

In: European Journal of Immunology, Vol. 24, No. 9, 09.1994, p. 2196-2202.

Research output: Contribution to journalArticle

Tanigaki, N, Fruci, D, Groome, N, Butler, RH, Londei, M & Tosi, R 1994, 'Exploring myelin basic protein for HLA class I-binding sequences', European Journal of Immunology, vol. 24, no. 9, pp. 2196-2202.
Tanigaki N, Fruci D, Groome N, Butler RH, Londei M, Tosi R. Exploring myelin basic protein for HLA class I-binding sequences. European Journal of Immunology. 1994 Sep;24(9):2196-2202.
Tanigaki, Nobuyuki ; Fruci, Doriana ; Groome, Niegel ; Butler, Richard H. ; Londei, Marco ; Tosi, Roberto. / Exploring myelin basic protein for HLA class I-binding sequences. In: European Journal of Immunology. 1994 ; Vol. 24, No. 9. pp. 2196-2202.
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