Exposure to 900 MHz Electromagnetic Field Induces an Unbalance between Pro-Apoptotic and Pro-Survival Signals in T-Lymphoblastoid Leukemia CCRF-CEM Cells

F. Marinelli, D. La Sala, G. Cicciotti, L. Cattini, C. Trimarchi, S. Putti, A. Zamparelli, L. Giuliani, G. Tomassetti, Caterina Cinti

Research output: Contribution to journalArticle

Abstract

It has been recently established that low-frequency electromagnetic field (EMFs) exposure induces biological changes and could be associated with increased incidence of cancer, whi lethe issue remains unresolved as to whether high-frequency EMFs can have hazardous effect on health. Epidemiological studies on association between childhood cancers, particularly leukemia and brain cancer, and exposure to low- and high-frequency EMF suggested an etiological role of EMFs in inducing adverse health effects. To investigate whether exposure to high-frequency EMFs could affect in vitro cell survival, we cultured acute T-lymphoblastoid leukemia cells (CCRF-CEM) in the presence of unmodulated 900 MHz EMF, generated by a transverse electromagnetic (TEM) cell, at various exposure times. We evaluated the effects of high-frequency EMF on cell growth rate and apoptosis induction, by cell viability (MTT) test, FACS analysis and DNA ladder, and we investigated proapoptotic and pro-survival signaling pathways possibly involved as a function of exposure time by Western blot analysis. At short exposure times (2-12 h), unmodulated 900 MHz EMF induced DNA breaks and early activation of both p53-dependent and -independent apoptotic pathways while longer continuous exposure (24-48 h) determined silencing of pro-apoptotic signals and activation of genes involved in both intracellular (Bcl-2) and extracellular (Ras and Akt1) prosurvival signaling. Overall our results indicate that exposure to 900 MHz continuous wave, after inducing an early self-defense response triggered by DNA damage, could confer to the survivor CCRF-CEM cells a further advantage to survive and proliferate.

Original languageEnglish
Pages (from-to)324-332
Number of pages9
JournalJournal of Cellular Physiology
Volume198
Issue number2
DOIs
Publication statusPublished - Feb 2004

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

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    Marinelli, F., La Sala, D., Cicciotti, G., Cattini, L., Trimarchi, C., Putti, S., Zamparelli, A., Giuliani, L., Tomassetti, G., & Cinti, C. (2004). Exposure to 900 MHz Electromagnetic Field Induces an Unbalance between Pro-Apoptotic and Pro-Survival Signals in T-Lymphoblastoid Leukemia CCRF-CEM Cells. Journal of Cellular Physiology, 198(2), 324-332. https://doi.org/10.1002/jcp.10425