Exposure to extremely low-frequency (50Hz) electromagnetic fields enhances adult hippocampal neurogenesis in C57BL/6 mice

Bruna Cuccurazzu, Lucia Leone, Maria Vittoria Podda, Roberto Piacentini, Elisa Riccardi, Cristian Ripoli, Gian Battista Azzena, Claudio Grassi

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Throughout life, new neurons are continuously generated in the hippocampus, which is therefore a major site of structural plasticity in the adult brain. We recently demonstrated that extremely low-frequency electromagnetic fields (ELFEFs) promote the neuronal differentiation of neural stem cells in vitro by up-regulating Cav1-channel activity. The aim of the present study was to determine whether 50-Hz/1 mT ELFEF stimulation also affects adult hippocampal neurogenesis in vivo, and if so, to identify the molecular mechanisms underlying this action and its functional impact on synaptic plasticity. ELFEF exposure (1 to 7h/day for 7days) significantly enhanced neurogenesis in the dentate gyrus (DG) of adult mice, as documented by increased numbers of cells double-labeled for 5-bromo-deoxyuridine (BrdU) and doublecortin. Quantitative RT-PCR analysis of hippocampal extracts revealed significant ELFEF exposure-induced increases in the transcription of pro-neuronal genes (Mash1, NeuroD2, Hes1) and genes encoding Cav1.2 channel α1C subunits. Increased expression of NeuroD1, NeuroD2 and Cav1 channels was also documented by Western blot analysis. Immunofluorescence experiments showed that, 30days after ELFEF stimulation, roughly half of the newly generated immature neurons had survived and become mature dentate granule cells (as shown by their immunoreactivity for both BrdU and NeuN) and were integrated into the granule cell layer of the DG. Electrophysiological experiments demonstrated that the new mature neurons influenced hippocampal synaptic plasticity, as reflected by increased long-term potentiation. Our findings show that ELFEF exposure can be an effective tool for increasing in vivo neurogenesis, and they could lead to the development of novel therapeutic approaches in regenerative medicine.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalExperimental Neurology
Volume226
Issue number1
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Electromagnetic Fields
Neurogenesis
Inbred C57BL Mouse
Deoxyuridine
Neuronal Plasticity
Dentate Gyrus
Neurons
Regenerative Medicine
Neural Stem Cells
Long-Term Potentiation
Genes
Fluorescent Antibody Technique
Hippocampus
Cell Count
Western Blotting
Polymerase Chain Reaction
Brain

Keywords

  • Ca1 channels
  • Dentate gyrus
  • Electromagnetic field
  • Long-term potentiation
  • Neural stem cells
  • NeuroD
  • Neurogenesis
  • Neuronal differentiation
  • Proliferation

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Medicine(all)

Cite this

Exposure to extremely low-frequency (50Hz) electromagnetic fields enhances adult hippocampal neurogenesis in C57BL/6 mice. / Cuccurazzu, Bruna; Leone, Lucia; Podda, Maria Vittoria; Piacentini, Roberto; Riccardi, Elisa; Ripoli, Cristian; Azzena, Gian Battista; Grassi, Claudio.

In: Experimental Neurology, Vol. 226, No. 1, 11.2010, p. 173-182.

Research output: Contribution to journalArticle

Cuccurazzu, Bruna ; Leone, Lucia ; Podda, Maria Vittoria ; Piacentini, Roberto ; Riccardi, Elisa ; Ripoli, Cristian ; Azzena, Gian Battista ; Grassi, Claudio. / Exposure to extremely low-frequency (50Hz) electromagnetic fields enhances adult hippocampal neurogenesis in C57BL/6 mice. In: Experimental Neurology. 2010 ; Vol. 226, No. 1. pp. 173-182.
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