Expression analysis and modulation by HIV-Tat of the tyrosine phosphatase HD-PTP

Massimo Mariotti, Sara Casliglioni, Jeanette A M Maier

Research output: Contribution to journalArticle

Abstract

The human immunodeficiency virus type 1 Tat transactivates viral proteins and also affects the expression of eukaryotic genes. Since Tat is angiogenic, we assumed that the isolation of differentially expressed genes in Tat-treated endothelial cells would yield insights into the molecular mechanisms of the angiogenic process. By RNA fingerprinting, we found that Tat upregulates the tyrosine phosphatase HD-PTP mRNA in a human endothelial cell line. At the moment, little is known about HD-PTP. We here show that HD-PTP is highly conserved through evolution from yeast to man, and is ubiquitously distributed in adult and fetal tissues. HD-PTP is expressed in human cell lines derived from different tumors, but the mRNA levels do not appear to correlate with the malignant phenotype of the cells. HD-PTP mRNA was also detected in ce 11 lines derived from tumors that develop in BKV/Tat-transgenicmice. Interestingly, a relation exists between the amounts of secreted Tat and the levels of HD-PTP mRNA. HD-PTP encodes a 185-kDa protein which is expressed in human endothelial from the umbilical cord and in human Kaposi-spindle cells. Tat-induction of HD-PTP mRNA parallels only with a slight increase of the protein, which occurs after 24 and 48 h of incubation in the presence of Tat. These results suggest that HD-PTP amounts might be regulated both at the transcriptional and post-transcriptional levels.

Original languageEnglish
Pages (from-to)301-308
Number of pages8
JournalJournal of Cellular Biochemistry
Volume98
Issue number2
DOIs
Publication statusPublished - May 15 2006

Keywords

  • AIDS
  • Angiogenesis
  • HD-PTP
  • Tat

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Expression analysis and modulation by HIV-Tat of the tyrosine phosphatase HD-PTP'. Together they form a unique fingerprint.

  • Cite this