Expression analysis of a subset of coregulators and three nuclear receptors in human colorectal carcinoma

Romina Giannini, Aldo Cavallini

Research output: Contribution to journalArticlepeer-review

Abstract

In human colorectal tissue samples, the gene expressions of 4 coactivators, p300, pCAF, TIF-2 and TRAP 220, and 7 corepressors, N-CoR, REA, MTA1, MTA1L1, HDAC1, HDAC2 and HDAC3, linked to estrogen receptors (ER), were revealed by traditional RT-PCR. Cofactors ERα, ERβ and ERRα mRNA levels were then measured in 40 tumor tissue samples matched with respective normal mucosa by real-time PCR. The decline of mRNA levels of all coactivators and the increase of NCoR, HDAC1, HDAC2 and MTA1 were observed from normal to tumor tissue, whereas REA, HDAC3 and MTA1L1 expressions were similar in both tissue compartments. The gene expression of ERβ correlated with those of p300, TIF-2 and REA in normal mucosa, and with that of REA in tumor tissue only. No association was found between ERα and coregulators and between each coregulator and different clinical parameters. Our findings suggest that the co-induction of ERβ and some cofactors may play an important role during the development of human colorectal carcinoma.

Original languageEnglish
Pages (from-to)4287-4292
Number of pages6
JournalAnticancer Research
Volume25
Issue number6 B
Publication statusPublished - Nov 2005

Keywords

  • Colorectal carcinoma
  • Coregulators
  • Estrogen receptor α
  • Estrogen receptor β
  • Estrogen receptor-related receptor α

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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