Expression and alternative splicing of fibronectin mRNA in human diploid endothelial cells during aging in vitro

Franco Pagani, Laura Zagato, Jeanette A M Maier, Giovanni Ragnotti, Domenico A. Coviello, Carlo Vergani

Research output: Contribution to journalArticle

Abstract

Different mRNAs for fibronectin arise from the variable processing of a single primary transcript. We used ribonuclease protection assay to investigate the changes occurring in fibronectin expression and the alternative splicing of mRNA precursor during aging in vitro of human diploid endothelial cells. Senescent endothelial cells release more protein and contain 4-5-fold more fibronectin mRNA than young cells. The pattern of alternative splicing of fibronectin mRNA, with the EDA and the CS1 segments largely included (35% and 77%, respectively) and the EDB segment undetectable, correlates well with previous studies at the protein level both in vitro and in vivo. No changes in the splicing pattern of fibronectin mRNA precursor were detected during endothelial cellular senescence. The increased expression of fibronectin in senescent cells may be a result of the activity of interleukin-1 α, which is overexpressed in senescent endothelial cells. It could be also important in vivo during aging and in atherosclerotic lesions.

Original languageEnglish
Pages (from-to)172-178
Number of pages7
JournalBBA - Gene Structure and Expression
Volume1173
Issue number2
DOIs
Publication statusPublished - May 28 1993

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Keywords

  • (Human)
  • Alternative splicing
  • Cellular senescence
  • Endothelial cell
  • Fibronectin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

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